I have solved it. The prgram mzXML2Search have been installed follow by TPP installed. Thank your very much for your kind.
Bright On 2月17日, 下午7时33分, Bill Nelson <[email protected]> wrote: > We were never able to switch one of our labs over to TPP because they > use ABI Q-TOF with Mascot and the Mascot daemon produced an MGF file > that searched much better that the MGF file produced by mzWiff & > Mascot2XML. Through postings on this forum we learned that the better > performance (about 20% more hits) was due to the spectrum averaging > algorithm used by the daemon. There are settable arguments available > in the mzWiff program for spectrum averaging but they don't seem to do > anything in our hands. Plus, in my opinion, the averaging should be > performed in the Mascot2XML program, leaving the mzXML to reflect the > original raw data. > Has anybody had any experience in addressing this issue with wiff to > mgf? > > Thanks, > Bill > > On Feb 16, 1:13 pm, Matthew Chambers <[email protected]> > wrote: > > > > > It depends what you want to use the MGF for. I think the TPP pipeline > > for the near future should continue using mxXML2Search for MGF output > > because of the way Mascot2XML expects the TITLE line to be formatted. In > > a future msconvert version, scan-number-only files like mzXML or Thermo > > RAW should convert to the DTA-style TITLE format that TPP is used to > > (except I don't think it does the 0-padding). On the other hand, if you > > don't care about using the MGF or its downstream products in the TPP, > > msconvert is easy to use for this purpose. > > > -Matt > > > Brian Pratt wrote: > > > The msconvert tool should handle that: > > > > To convert foo.mzxml to foo.mgf > > > > msconvert --mgf foo.mzxml > > > > -----Original Message----- > > > From: [email protected] > > > [mailto:[email protected]] On Behalf Of Bright > > > Sent: Monday, February 16, 2009 6:20 AM > > > To: spctools-discuss > > > Subject: [spctools-discuss] How to convert mzXML file to mgf file > > > > Hi everyone > > > I can't deal with the problem that convert mzXML file to mgf file. > > > Could your give me some ideal to over it. > > > Thank. --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [email protected] For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en -~----------~----~----~----~------~----~------~--~---
