I have solved it. The prgram mzXML2Search have been installed follow by TPP installed. Thank your very much for your kind.
Bright On 2月17日, 下午10时04分, Matt Chambers <[email protected]> wrote: > Do you mean mzXML2Search instead of Mascot2XML? I agree that spectrum > averaging should be a more universal feature; it's something I plan to > put in msconvert but haven't gotten around to yet. What range of > settings did you try for spectrum averaging in mzWiff? > > -Matt > > > > Bill Nelson wrote: > > We were never able to switch one of our labs over to TPP because they > > use ABI Q-TOF with Mascot and the Mascot daemon produced an MGF file > > that searched much better that the MGF file produced by mzWiff & > > Mascot2XML. Through postings on this forum we learned that the better > > performance (about 20% more hits) was due to the spectrum averaging > > algorithm used by the daemon. There are settable arguments available > > in the mzWiff program for spectrum averaging but they don't seem to do > > anything in our hands. Plus, in my opinion, the averaging should be > > performed in the Mascot2XML program, leaving the mzXML to reflect the > > original raw data. > > Has anybody had any experience in addressing this issue with wiff to > > mgf? > > > Thanks, > > Bill > > > On Feb 16, 1:13 pm, Matthew Chambers <[email protected]> > > wrote: > > >> It depends what you want to use the MGF for. I think the TPP pipeline > >> for the near future should continue using mxXML2Search for MGF output > >> because of the way Mascot2XML expects the TITLE line to be formatted. In > >> a future msconvert version, scan-number-only files like mzXML or Thermo > >> RAW should convert to the DTA-style TITLE format that TPP is used to > >> (except I don't think it does the 0-padding). On the other hand, if you > >> don't care about using the MGF or its downstream products in the TPP, > >> msconvert is easy to use for this purpose. > > >> -Matt > > >> Brian Pratt wrote: > > >>> The msconvert tool should handle that: > > >>> To convert foo.mzxml to foo.mgf > > >>> msconvert --mgf foo.mzxml > > >>> -----Original Message----- > >>> From: [email protected] > >>> [mailto:[email protected]] On Behalf Of Bright > >>> Sent: Monday, February 16, 2009 6:20 AM > >>> To: spctools-discuss > >>> Subject: [spctools-discuss] How to convert mzXML file to mgf file > > >>> Hi everyone > >>> I can't deal with the problem that convert mzXML file to mgf file. > >>> Could your give me some ideal to over it. > >>> Thank. --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [email protected] For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en -~----------~----~----~----~------~----~------~--~---
