Antonio,

That warning about not being able to open the mzXML file is not 
indicative of the underlying problem.  It is truly just a warning and 
the analysis process is capable of finishing correctly even if the 
corresponding mzXML file is missing.

The important question that's not clear from your description below is 
how did you run the Sequest search?  Did you run it separately via 
Bioworks or did you run the search through the TPP?  Although helpful 
but not required, it doesn't seem like you did the latter.  Do you have 
a directory (e.g. c:/Inetpub/wwwroot/ISB/data/AAS/test/) that has the 
Sequest .out files in it?

- Jimmy

Antonio wrote:
> I have installed TPP v4.2 on windows XP. I have created the mzXML
> datafiles from my Sequest search. Now I'm making the corresponding
> pepXML files. I see that the first file is being created, it seems
> that is finished but I see the following warming message at the end of
> the “Output so far” field:
> 
> warning: cannot open mzXML file "c:/Inetpub/wwwroot/ISB/data/AAS/
> test.mzXML" for reading MS instrument info and scan times.
> And the command status window indicates that the command is still
> running. Is it? I do not see the file increasing in size. I can open
> the pepxml file, although IE indicates that there is an error on page.
> It seems to me that the program is stuck and is not progressing to
> next task: the creation of the next pepXML file. Solutions?
> 
> Thanks,
> 
>  Antonio
> 
> > 


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