Hi Jimmy, I run Sequest from Bioworks on our computer cluster, and then transfer the files to the local machine running TPP, so yeas I had the c:/ Inetpub/wwwroot/ISB/data/AAS/test/ With the out-and dta- files in it. I have attempted to run Sequest from TPP and I had the same problem; the dta and out files are created, but the command indicates that is still running. The problem occurs whether I send a few files or a single one. But I have more than one file on the queue, the command will not proceed to the next file. I can run Sequest from Bioworks on the local machine without any problems.
On Jul 2, 6:55 pm, Jimmy Eng <[email protected]> wrote: > Antonio, > > That warning about not being able to open the mzXML file is not > indicative of the underlying problem. It is truly just a warning and > the analysis process is capable of finishing correctly even if the > corresponding mzXML file is missing. > > The important question that's not clear from your description below is > how did you run the Sequest search? Did you run it separately via > Bioworks or did you run the search through the TPP? Although helpful > but not required, it doesn't seem like you did the latter. Do you have > a directory (e.g. c:/Inetpub/wwwroot/ISB/data/AAS/test/) that has the > Sequest .out files in it? > > - Jimmy > > > > Antonio wrote: > > I have installed TPP v4.2 on windows XP. I have created the mzXML > > datafiles from my Sequest search. Now I'm making the corresponding > > pepXML files. I see that the first file is being created, it seems > > that is finished but I see the following warming message at the end of > > the “Output so far” field: > > > warning: cannot open mzXML file "c:/Inetpub/wwwroot/ISB/data/AAS/ > > test.mzXML" for reading MS instrument info and scan times. > > And the command status window indicates that the command is still > > running. Is it? I do not see the file increasing in size. I can open > > the pepxml file, although IE indicates that there is an error on page. > > It seems to me that the program is stuck and is not progressing to > > next task: the creation of the next pepXML file. Solutions? > > > Thanks, > > > Antonio- Hide quoted text - > > - Show quoted text - --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [email protected] For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en -~----------~----~----~----~------~----~------~--~---
