Hi Natalie

Thank you for your reply.

I am not using the command-line version.  I had downloaded the full
TPP on windows but when I try to convert the .raw files to .mzXML in
the GUI version, it complains of '--mzXML unknown option'.

I think whats happening is that ReAdW will work only if you run it in
the directory that its located in, but if you try to run it in another
directory it doesn't work.  However if I try to run it in any
directory using the command:  'ReAdW datafile name c', it works, but
doesn't generate the mzXML file.  I suppose what I want to know is
this: When the GUI version runs the command 'ReAdW etc' where does it
read the ReAdW file from?  In another words, How can I know the path
from which the GUI runs ReAdW?

Thanks

Ali

On Jul 7, 6:02 pm, Natalie Tasman <[email protected]>
wrote:
> Hello Ali,
> It sounds like you're using the command-line version of the converter
> ("ReAdW").  The easiest way to get started with the TPP on Windows is
> to download the complete TPP installer, which will set up and
> configure a web-based interface.  This interface should be a lot
> easier to use.
>
> If you do want to stick with the command-line version, you just need
> to type "readw.exe --mzXML <your filename.raw>",
> to specify mzXML output.
>
> Best wishes, and please let us know how it goes,
>
> Natalie
>
> On Tue, Jul 7, 2009 at 4:39 AM, Ali <[email protected]> wrote:
>
> > Hi
>
> > I'm new to TPP.  I downloaded the software and attempted to convert
> > XCalibur .RAW to mzXML files as the first step of the pipeline.  This
> > however failed as it complains of '--mzXML unknown option'.  I checked
> > in my tpp-bin file that I have the ReAdW file.  I used the ReAdW in
> > dos and it converted the .RAW file but it doesn't seem to work in
> > TPP.  Any advice will be appreciated.

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