The m/z and intensity of each transition at each scan is stored in that scan's peak list in base64. So it's not human readable, but it's there. Incidentally, the mzML format stores SRM data in a chromatogram-centric way which is far more space and time efficient. Depending on what tool you're using to analyze the data you might try the new format.
-Matt Harry wrote: > Hello group members! > > I encountered a problem when using the data conversion function in the > TTP (v4.2 JETSTREAM rev 1, Build 200904071723) to convert my MRM > experimental data in .RAW format to mzXML. > > In my MRM experiment setup, I had 3 transitions (i.e. 3 fragment ions) > for each precursor ion. In the mzXML file that was converted from the > RAW data file, for each precursor ion, it showed the "base peak > intensity" and "total ion current", which is the signal intensity of > the strongest transition and the sum of the signal intensity for all 3 > transitions, respectively. What is also very important for my > experiment, but missing in the mzXML file, is the signal intensity of > the weakest transition for each precursor ion. > > So the question is: How can I get the individual signal intensity for > all 3 transitions of a precursor ion to show up in the mzXML file that > is converted from a RAW data file acquired in a MRM experiment? > > Thanks so much for your help! > > Harry > > --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [email protected] For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en -~----------~----~----~----~------~----~------~--~---
