Hello Matt, Thanks for replying to my question! I converted the my RAW date file to mzML, but had problems opening the mzML file in Excel. When I tried to open the file as an XML list, I got the message: "There is a problem with the specified XML or schema source". I clicked OK, then got another error message: "XML input Error: some data failed to import". In the end I got the spread sheet with column heads, but no actual data. I also tried to open the file as a read-only workbook, again got an error message, even though this time I can see part of the data appeared in the spread sheet. When I clicked OK on the error message, all was lost in the spread sheet.
Could you enlighten me what might be the cause of this problem and how to open the mzML files? P.S. you mentioned that mzML format stores SRM data in a chromatogram- centric way, my data were collected using static infusion. I wonder if that would cause any problem. Thanks a lot, Harry On Aug 6, 8:22 am, Matthew Chambers <[email protected]> wrote: > The m/z and intensity of each transition at each scan is stored in that > scan's peak list in base64. So it's not human readable, but it's there. > Incidentally, the mzML format stores SRM data in a chromatogram-centric > way which is far more space and time efficient. Depending on what tool > you're using to analyze the data you might try the new format. > > -Matt > > Harry wrote: > > Hello group members! > > > I encountered a problem when using the data conversion function in the > > TTP (v4.2 JETSTREAM rev 1, Build 200904071723) to convert my MRM > > experimental data in .RAW format to mzXML. > > > In my MRM experiment setup, I had 3 transitions (i.e. 3 fragment ions) > > for each precursor ion. In the mzXML file that was converted from the > > RAW data file, for each precursor ion, it showed the "base peak > > intensity" and "total ion current", which is the signal intensity of > > the strongest transition and the sum of the signal intensity for all 3 > > transitions, respectively. What is also very important for my > > experiment, but missing in the mzXML file, is the signal intensity of > > the weakest transition for each precursor ion. > > > So the question is: How can I get the individual signal intensity for > > all 3 transitions of a precursor ion to show up in the mzXML file that > > is converted from a RAW data file acquired in a MRM experiment? > > > Thanks so much for your help! > > > Harry --~--~---------~--~----~------------~-------~--~----~ You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected] To unsubscribe from this group, send email to [email protected] For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en -~----------~----~----~----~------~----~------~--~---
