Hello Andrew,

Please refer to my previous reply to your questions (topic: "ReAdW DLL  
installation location").

In short: ReAdW is not based on Extract_MSN.  It is a different  
program and has different requirements than Mascot Daemon (which comes  
from Matrix Science, not the TPP).  Rather, ReAdW requires different  
parts of Thermo's Xcalibur software.  Do note that you may have to  
install the Xcalibur "XDK".

Again, please find my recommendations regarding moving forward with  
the newer msconvert program, which requires yet another, but free,  
piece of Thermo software that you can download and install to any  
computer.

-Natalie


On Sep 15, 2009, at 2:53 PM, AndrewK wrote:

>
> I have a version of Bioworks (Sequest) that uses Extract_MSN (lcq.dta)
> executable(s) and these dll files to generate searchable dta files:
>
> FControl2.dll
> Fglobal.dll
> Fileio.dll
> UnifiedFile.dll
>
> For Mascot Daemon to feed searchable files to the local Mascot Server,
> all I have to do is place these files in any directory on the computer
> and tell Mascot Daemon where the files are (C:\path\path...etc).
> The .RAW files are converted using the Extract_MSN program, and
> searchable data is sent to the Mascot server.
>
> A standard installation of Sequest (Bioworks) puts these files in the
> directory called
> C:\Xcalibur\System\Programs
>
> I tried generating these directories and put the files there Sequest
> is not loaded on this computer), but ReAdW is still failing.
>
> I've done this on many workstations to allow Mascot Daemon to convert
>> RAW files to dta files (expected input format), and it always works.
>
> Where do I need to place these files so that TPP finds them?
>
> Andrew
> >


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