Hallo,

to my knowledge Proteome Discoverer 1.0 is not supporting batch 
processing yet. Should come with 1.1.

Dominik

Nikhil Garge wrote:
> Hello,
> Anybody has experience using "Proteome Discoverer 1.0" software for 
> SEQUEST search. I use the Bioworks for SEQUEST search and new to 
> "Proteome Discoverer 1.0". few of my concerns are as follows:
> a) How to perform SEQUEST BATCH search using "Proteome Discoverer 
> 1.0"? It seems to accept only ine raw file at a time. Do you know how 
> can one supply multiple raw files for SEQEUST search?
> b) The output is *.msf file which can be exported into protXML file 
> format. So does this mean "Proteome Discoverer 1.0" uses Protein 
> Prophet algorithm to identify significant proteins?
> c) The SEQUEST search done through "Proteome Discoverer 1.0" doesnt 
> seem to output *.out files (or pepXML files). I am more interested in 
> individual peptide hits but dont see how one can get 1st and 2nd hit 
> as we see in *.out file using Bioworks).
> I would really appreciate your feedback on this.
> Thanks,
> ~Nikhil.
>
> >


-- 
Dr. Dominik Schwudke
Group Leader

National Centre for Biological Sciences
Tata Institute of Fundamental Research
GKVK, Bellary Road,
Bangalore 560065, India

Phone +91-80 – 23666499
[email protected]


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