Hi Surendra,
The warning indicates that the correct modification specifications
were not written as <aminoacid_modification> tags to the
<search_summary> section of the pepXML.  I guess just writing the
<mod_aminoacid_mass> tags in the <search_hits> might be an improvement
over just dropping them, but the real solution should be to figure out
why the <aminoacid_modification> tags aren't getting written.

Is there anything in the X! Tandem parameters than can be read as
indicating that these modifications are possible?  Usually there is.

If you don't write internally consistent modifications, some tools
like CPAS (on which I was working when I wrote Tandem2XML) will have
difficulty consuming the resulting pepXML.  I am sure Jimmy Eng or
Brian Pratt (included on this message) can help you connect with
people currently working on the TPP.

--Brendan

On Dec 10, 9:24 am, dasaris <[email protected]> wrote:
> Hi There,
>
>                I noticed an issue in Tandem2XML program. When the
> program encounters a modification that is not in input parameters, it
> issues an "unknown modification" warning and ignores it. However, this
> often happens when people search for point mutations with X! Tandem. I
> fixed the program such that it will write any unknown modifications to
> the pepXML instead of ignoring them. Let me know if you are interested
> in getting the fix into your source tree.
>
> Thanks,
> Surendra

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