Hi everybody, currently I am trying to work my way through TPP. Subsequent to converting my Thermo .raw files to mzXML I tried to do a Sequest search with these files, using a decoy database that I generated with the TPP tool Decoy Database. Conversion seemed to work properly, however, when I start the search I immediately get the error message "Cannot read the database file '/dbase/IPI/ ipi.MOUSE.v3.64_DECOY.fasta'. Check that this filename is correct.". The file name appears to be correct, the file is located where it is supposed to be, the database is specified in the params file, yet.... Does anybody have a clue on what might have gone wrong?
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