No but it might help in debugging. You say TPP can't see the database file in '/dbase/IPI/' which could mean that you store all your fasta files in a directory called /dbase on a linux system. Alternatively, this could be on a Windows system running cygwin. I couldn't tell based on the error message. I think Sequest file paths on Windows should look like this: C:\dbase \IPI\ipi.MOUSE.v3.64_DECOY.fasta NOT like what you posted: '/dbase/IPI/ipi.MOUSE.v3.64_DECOY.fasta'
Get the full path to your fasta file using Windows Explorer and use that path in Sequest. HTH, GATTACA On Dec 22, 3:42 am, hcadrexler <[email protected]> wrote: > I am running Windows XP 32-bit - would it make a difference? > > On 21 Dez., 16:53, GATTACA <[email protected]> wrote: > > > What OS are you running? Windows? Linux? > > > On Dec 21, 3:54 am, hcadrexler <[email protected]> wrote: > > > > Hi everybody, > > > > currently I am trying to work my way through TPP. Subsequent to > > > converting my Thermo .raw files to mzXML I tried to do a Sequest > > > search with these files, using a decoy database that I generated with > > > the TPP tool Decoy Database. Conversion seemed to work properly, > > > however, when I start the search I immediately get the error message > > > "Cannot read the database file '/dbase/IPI/ > > > ipi.MOUSE.v3.64_DECOY.fasta'. Check that this filename is correct.". > > > The file name appears to be correct, the file is located where it is > > > supposed to be, the database is specified in the params file, yet.... > > > Does anybody have a clue on what might have gone wrong? -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
