Hi HTH,
you may be right - I will have a closer look at the path. Thanks for
your help! Happy Holidays!

Hannes

2009/12/22 GATTACA <[email protected]>:
> No but it might help in debugging. You say TPP can't see the database
> file in '/dbase/IPI/' which could mean that you store all your fasta
> files in a directory called /dbase on a linux system. Alternatively,
> this could be on a Windows system running cygwin. I couldn't tell
> based on the error message.
> I think Sequest file paths on Windows should look like this: C:\dbase
> \IPI\ipi.MOUSE.v3.64_DECOY.fasta
> NOT like what you posted: '/dbase/IPI/ipi.MOUSE.v3.64_DECOY.fasta'
>
> Get the full path to your fasta file using Windows Explorer and use
> that path in Sequest.
>
> HTH,
> GATTACA
>
>
> On Dec 22, 3:42 am, hcadrexler <[email protected]> wrote:
>> I am running Windows XP 32-bit - would it make a difference?
>>
>> On 21 Dez., 16:53, GATTACA <[email protected]> wrote:
>>
>> > What OS are you running? Windows? Linux?
>>
>> > On Dec 21, 3:54 am, hcadrexler <[email protected]> wrote:
>>
>> > > Hi everybody,
>>
>> > > currently I am trying to work my way through TPP. Subsequent to
>> > > converting my Thermo .raw files to mzXML I tried to do a Sequest
>> > > search with these files, using a decoy database that I generated with
>> > > the TPP tool Decoy Database. Conversion seemed to work properly,
>> > > however, when I start the search I immediately get the error message
>> > > "Cannot read the database file '/dbase/IPI/
>> > > ipi.MOUSE.v3.64_DECOY.fasta'.  Check that this filename is correct.".
>> > > The file name appears to be correct, the file is located where it is
>> > > supposed to be, the database is specified in the params file, yet....
>> > > Does anybody have a clue on what might have gone wrong?
>
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