Dave, Matt,
Thanks for the comments.

My Windows happens to be living in a Virtualbox on a Mac Pro, so that
explains the overall sluggish pace! I guess that's part of the fun of
needing the Agilent library.

Out of curiosity, I might try running one of the jobs on my own year-
old laptop, just in case this is data related..

Cheers,
bio.x2y

On Feb 16, 9:26 pm, Matthew Chambers <[email protected]>
wrote:
> The delay in start up time for Agilent is a known issue. Unfortunately
> the current Agilent API doesn't provide a way to get the list of scanIds
> in a data file, nor a way to get a spectrum's scanId without getting its
> data arrays. ProteoWizard is designed to support random access by
> nativeID and by index to all of the data formats it supports, so it has
> to enumerate all the spectra up front in order to get each of their
> scanIds. On profile data, that takes a frustrating amount of time.
>
> I have a feature request pending with Agilent to get a function which
> provides either a list of scanIds or a spectrum without metadata.
>
> Thanks for doing the comparison. I agree with Dave that the conversion
> time sounds pretty long in both cases and I suspect a network share.
>
> -Matt
>
>
>
> bio.x2y wrote:
> > Hi,
>
> > I independently used both Trapper (4.3.1) and Msconvert (pwiz 1.6.0)
> > to convert a 1.4Gb Agilent MassHunter ".d" file containing ~16000
> > spectra to mzXML.
>
> > Parameters:
> > $msconvert --mzXML --verbose large.d
> > $trapper --mzXML -v large.d large.mzXML
>
> > Msconvert took 5hrs 38mins to complete, generating a 57Gb file.
> > Trapper took 1hr 26mins to complete, generating a 28Gb file.
>
> > I can understand the differences in size, given the differences in
> > structure and precision. However, the time difference still appears
> > quite high.
>
> > One interesting observation is that msconvert does not start writing
> > to the output file until 1hr 20min has elapsed. At that point, the
> > file begins filling and the progress messages start appearing in the
> > output. Trapper, on the other hand, starts filling the output file and
> > reporting progress immediately.
>
> > I have seen this occur now for two runs on two different days, so I
> > don't think it's related to other activity on the machine.
>
> > Perhaps msconvert is engaging in some preprocessing that isn't
> > strictly necessary, for Agilent ".d" files at least?
>
> > Thanks,
> > bio.x2y

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