Matt,

Thanks for the explanation.   Does this mean that trapper isn't using the
Agilent API? (Asking the naive trapper user question)

-Joe


On Tue, Feb 16, 2010 at 1:26 PM, Matthew Chambers <
[email protected]> wrote:

> The delay in start up time for Agilent is a known issue. Unfortunately the
> current Agilent API doesn't provide a way to get the list of scanIds in a
> data file, nor a way to get a spectrum's scanId without getting its data
> arrays. ProteoWizard is designed to support random access by nativeID and by
> index to all of the data formats it supports, so it has to enumerate all the
> spectra up front in order to get each of their scanIds. On profile data,
> that takes a frustrating amount of time.
>
> I have a feature request pending with Agilent to get a function which
> provides either a list of scanIds or a spectrum without metadata.
>
> Thanks for doing the comparison. I agree with Dave that the conversion time
> sounds pretty long in both cases and I suspect a network share.
>
> -Matt
>
>
>
> bio.x2y wrote:
>
>> Hi,
>>
>> I independently used both Trapper (4.3.1) and Msconvert (pwiz 1.6.0)
>> to convert a 1.4Gb Agilent MassHunter ".d" file containing ~16000
>> spectra to mzXML.
>>
>> Parameters:
>> $msconvert --mzXML --verbose large.d
>> $trapper --mzXML -v large.d large.mzXML
>>
>> Msconvert took 5hrs 38mins to complete, generating a 57Gb file.
>> Trapper took 1hr 26mins to complete, generating a 28Gb file.
>>
>> I can understand the differences in size, given the differences in
>> structure and precision. However, the time difference still appears
>> quite high.
>>
>> One interesting observation is that msconvert does not start writing
>> to the output file until 1hr 20min has elapsed. At that point, the
>> file begins filling and the progress messages start appearing in the
>> output. Trapper, on the other hand, starts filling the output file and
>> reporting progress immediately.
>>
>> I have seen this occur now for two runs on two different days, so I
>> don't think it's related to other activity on the machine.
>>
>> Perhaps msconvert is engaging in some preprocessing that isn't
>> strictly necessary, for Agilent ".d" files at least?
>>
>> Thanks,
>> bio.x2y
>>
>>
>>
>
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-- 
Joe Slagel
Institute for Systems Biology
[email protected]
(206) 732-1362

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