Hi,

we are using an Agilent MSD/TOF for metabolomics. For us, it is
important to convert to CENTROID mzXML for further data processing,
otherwise the files become extremely large.

Greetings, Robert


On Feb 16, 2:52 pm, "bio.x2y" <[email protected]> wrote:
> Hi,
>
> I independently used both Trapper (4.3.1) and Msconvert (pwiz 1.6.0)
> to convert a 1.4Gb Agilent MassHunter ".d" file containing ~16000
> spectra to mzXML.
>
> Parameters:
> $msconvert --mzXML --verbose large.d
> $trapper --mzXML -v large.d large.mzXML
>
> Msconvert took 5hrs 38mins to complete, generating a 57Gb file.
> Trapper took 1hr 26mins to complete, generating a 28Gb file.
>
> I can understand the differences in size, given the differences in
> structure and precision. However, the time difference still appears
> quite high.
>
> One interesting observation is that msconvert does not start writing
> to the output file until 1hr 20min has elapsed. At that point, the
> file begins filling and the progress messages start appearing in the
> output. Trapper, on the other hand, starts filling the output file and
> reporting progress immediately.
>
> I have seen this occur now for two runs on two different days, so I
> don't think it's related to other activity on the machine.
>
> Perhaps msconvert is engaging in some preprocessing that isn't
> strictly necessary, for Agilent ".d" files at least?
>
> Thanks,
> bio.x2y

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