Hi David,

Thank you very much!

Rachel :)

On Sep 30, 2:46 am, David Shteynberg <[email protected]>
wrote:
> Hi Rachel,
>
> It is limited only by the amout of memory you have on your system.
> The memory req  scales linearly with number of spectra being combined.
>   Obviously the more spectra that are analysed together the better the
> statistics are going to be, but this too has a practical limit.  Once
> you have the individual iProphet pepxmls you should not run iProphet
> on them again since you'll run into the same memory issue.  Instead
> this is a perfect time to run ProteinProphet on the iProphet files and
> specify the IPROPHET option to make sure it uses iProphet
> probabilities.
>
> -David
>
>
>
> On Tue, Sep 28, 2010 at 7:23 PM, Rachel <[email protected]> wrote:
> > 1. I tried to combine large number of files (~1000) using iprophet. I
> > tried using command line as well as the tpp-gui.  but failed.
> > The error message is,
> > This application has requested the runtime to terminate in an unusual
> > way. Please contact the application's team for more information.
>
> > 2. Then I split the 1000 files into 12 batches and combine the
> > peptideprophet of these files using iprophet. Then I tried to combine
> > these 12 large iprophet files in iprophet, also failed. I got the same
> > error message.
>
> > Is anyone know the limit of iProphet?
>
> > thanks a lot.
>
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