No data compression on the peak list?

Try running the file through mscovert, it does not use the RAMP parser
and should give a good sanity check on the mzXML file in question.

Instead of guessing at what's reasonable, I'd advise determining what
the actual value is (again, msconvert could help here, use it to
produce a human readable representation like MGF).

On Thu, Feb 3, 2011 at 1:23 AM, dre <[email protected]> wrote:
> I haven't tried any yet, but I was assuming most would be based on the
> same ramp libraries? Actually, I'll give you an example of what I'm
> doing, maybe you can help point out any mistakes.
>
> I have a mzxml file with the following attributes (among others):
> <mzXML xmlns="http://sashimi.sourceforge.net/schema_revision/
> mzXML_3.0"
> <software type="conversion" name="ReAdW" version="4.2.1(build Apr  7
> 2009 13:17:55)" />
> <peaks precision="32"
>    byteOrder="network"
>    pairOrder="m/z-int" > Q74ZWUQe5SJDvypeQ8tdo0PAk6BEh1/bQ
> (continued, but I'll only post the first few chars for simplicity)
>
> So I'll consider making the first float out of the first few chars.
> I'll do manually the base64 decoding then to binary
>
> Q   7    4    Z     W    U
> 16  59  56   25   22    20
> 010000 111011 111000 011001 010110 010100 (binary)
> 43BE1959 (hex)
>
> then if I directly convert this to a float I get:
>
> 43BE1959 = 380.19803 float
>
> which is a reasonable number. But if I assume the data starts in
> network byte order, I would first have to do a network rearrangement
> of the bytes and then a float representation:
>
> 43BE1959 (assumed network) to host 5919be43
>
> then to float
>
> 5919be43 = 2.70467 E15 float
>
> So where am I going wrong?
>
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