I am running the TPP on a windows 7 machine.

I used the command line to convert (using msconvert) a .mgf file to
a .mzXML format for processing.

I have examined the resulting mzXML file from the conversion and it
contains what appears to be valid data and has msLevel=2 included so I
am certain it is tandem.  I did not run the experiment myself so I
cannot be certain of the protocol implemented.

I am using a FASTA format database to search against and I am using a
default tandem parameters file.  When I attempt to execute the X!
Tandem search of the data, I receive the following error:

 run_in c:/Inetpub/wwwroot/ISB/data/Test; c:\Inetpub\tpp-bin\tandem c:/
Inetpub/wwwroot/ISB/data/Test/test.tandem.params

X! TANDEM 2009.10.01.1 (LabKey and ISB)

Loading spectra . loaded.
No input spectra met the acceptance criteria.

command "c:\Inetpub\tpp-bin\tandem c:/Inetpub/wwwroot/ISB/data/Test/
test.tandem.params" failed: Operation not permitted

Command FAILED

Any thoughts on the cause?
Thanks in advance.

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