How did you examine the file? Did you try to visualize it with Pep3D or the Insilicos Viewer? Are there good peaks in the ms2 spectra? XTandem has a number of peaks threshold for analyzing a spectrum; perhaps none pass this limit?
On Thu, Jul 7, 2011 at 1:38 PM, Colin <[email protected]> wrote: > I am running the TPP on a windows 7 machine. > > I used the command line to convert (using msconvert) a .mgf file to > a .mzXML format for processing. > > I have examined the resulting mzXML file from the conversion and it > contains what appears to be valid data and has msLevel=2 included so I > am certain it is tandem. I did not run the experiment myself so I > cannot be certain of the protocol implemented. > > I am using a FASTA format database to search against and I am using a > default tandem parameters file. When I attempt to execute the X! > Tandem search of the data, I receive the following error: > > run_in c:/Inetpub/wwwroot/ISB/data/Test; c:\Inetpub\tpp-bin\tandem c:/ > Inetpub/wwwroot/ISB/data/Test/test.tandem.params > > X! TANDEM 2009.10.01.1 (LabKey and ISB) > > Loading spectra . loaded. > No input spectra met the acceptance criteria. > > command "c:\Inetpub\tpp-bin\tandem c:/Inetpub/wwwroot/ISB/data/Test/ > test.tandem.params" failed: Operation not permitted > > Command FAILED > > Any thoughts on the cause? > Thanks in advance. > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To post to this group, send email to [email protected]. > To unsubscribe from this group, send email to > [email protected]. > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
