Hi, I noticed that peptides containing consecutive R's or K's are given 1 missed cleavage, and 0 if they're N or C-terminal. For example R.ADSLIPMRRR.K would have 0 missed cleavages and R.ADSLIRRRM.K would have 1 missed cleavage. Is it known that trypsin typically only cleaves once in a sequence of consecutive R/Ks or is there some other reason? I'm viewing the data through the PeptideProphet viewer using TPP 4.5.
Thank you, -- Dennis Goldfarb Graduate Student Department of Computer Science University of North Carolina at Chapel Hill -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
