Problem solved...
Ron from X!Tandem replied to my question and he suggested to switch of
the refinement steps, which indeed helped:
 <note label="refine" type="input">no</note> "

Best
Olga

On Nov 21, 10:43 am, olgas <[email protected]> wrote:
> I wanted to use X!Tandem for one of my searches but I realised that it
> is not possible to choose 0 missed cleavages. There is the "scoring,
> maximum missed cleavage sites" parameter but this has to be ≥1 and
> can't be 0.
> Is there a way to search only for peptides with 0 missed cleavages?
>
> Thanks a lot for any comment/help!

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