Hi there, I'm running some dimethylaiton experiments with light (+28.0313@K and nt) and heavy (+32.0564@K and nt) labelling. I've read prior posts that recommend doing two static searches (using XTandem) for both light and heavy modifications and then run xinteract. Now I'm completing the Xpress and ASAPratio on Petunia UI and I'm a little stuck on the parameters to fill, specifically parameters: Change XPRESS residue mass difference AND Specified residue mass (For Asap). Do I need to fill in these values, or will they be picked up from Xtandem results? Should I fill the values for K and nt again or are they picked up from the Xtandem results? I'm a new TPP user and WILL APRECIATE ANY HELP!!!
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