Is it getting stuck at "PepXMLViewer.cgi"...

I am not sure if it will help... do you have "tmp" folder under "C:" drive ?

On Mon, Jul 30, 2012 at 7:33 PM, zeyu sun <[email protected]> wrote:

>
> Dear TPP team:
> I came across a error message that I really cannot explain why. Hopefully 
> some of experts here can help me to decode the message. Really appreciated!
> The data was collected from a LTQ-Orbitrap, the search was done by X!tandem 
> within the TPP environment using the default setting 
> "isb_default_input_kscore setting".
>
> The search result was automatically converted to pep.xml and when I try to 
> filter the results with peptideprophet I got error message like this:
> _____________________________________________________________________________________________________________________________
>
>  run_in c:/Inetpub/wwwroot/ISB/data/data/AIF/20120728/processed; 
> c:\Inetpub\tpp-bin\xinteract  -Nbsa1_3con_filtered.pep.xml -p0.95 -l7 -O 
> bsa1_3con.tandem.pep.xml
>
> ------------------------------
>
> c:\Inetpub\tpp-bin\xinteract (TPP v4.5 RAPTURE rev 2, Build 201202031108 
> (MinGW))
>
> running: "C:/Inetpub/tpp-bin/InteractParser "bsa1_3con_filtered.pep.xml" 
> "bsa1_3con.tandem.pep.xml" -L"7""
>  file 1: bsa1_3con.tandem.pep.xml
>  processed altogether 4615 results
>
>
>  results written to file 
> c:/Inetpub/wwwroot/ISB/data/data/AIF/20120728/processed/bsa1_3con_filtered.pep.xml
>
>  direct your browser to 
> http://localhost/ISB/data/data/AIF/20120728/processed/bsa1_3con_filtered.pep.shtml
>
>
>
> command completed in 1 sec
>
> running: "C:/Inetpub/tpp-bin/DatabaseParser "bsa1_3con_filtered.pep.xml""
> command completed in 1 sec
>
> running: "C:/Inetpub/tpp-bin/RefreshParser "bsa1_3con_filtered.pep.xml" 
> "c:/Inetpub/wwwroot/ISB/data/dbase/BOVIN_DECOY.fasta""
>
>
> ------------------------------
> * ...skipping 23 lines of output... *
> ------------------------------
>
> WARNING: Mixture model quality test failed for charge (3+).
>   model complete after 25 iterations
> command completed in 1 sec
>
> running: "C:/Inetpub/tpp-bin/ProphetModels.pl -i bsa1_3con_filtered.pep.xml"
> Analyzing bsa1_3con_filtered.pep.xml ...
> Parsing search results 
> "c:/Inetpub/wwwroot/ISB/data/data/AIF/20120728/processed/bsa1_3con (X! Tandem 
> (k-score))"...
>   => Total of 0 hits.
>   => Total of 0 decoy hits.
>   => Total of 0 excluded hits.
> command completed in 0 sec
>
> running: "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I 
> c:/Inetpub/wwwroot/ISB/data/data/AIF/20120728/processed/bsa1_3con_filtered.pep.xml"
>
> command "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I 
> c:/Inetpub/wwwroot/ISB/data/data/AIF/20120728/processed/bsa1_3con_filtered.pep.xml"
>  failed: Unknown error
>
> command "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I 
> c:/Inetpub/wwwroot/ISB/data/data/AIF/20120728/processed/bsa1_3con_filtered.pep.xml"
>  exited with non-zero exit code: 255
> QUIT - the job is incomplete
>
> command "c:\Inetpub\tpp-bin\xinteract -Nbsa1_3con_filtered.pep.xml -p0.95 -l7 
> -O bsa1_3con.tandem.pep.xml" failed: Unknown error
>
> *Command FAILED
> __________________________________________________________
>
> thank you so much in advance!
>
> sunsun
> *
>
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