Thanks for the reply Vishal,
Yes, I do have tmp folder under the C drive.
SunSun

On Tue, Jul 31, 2012 at 12:13 PM, Vishal Swaroop <[email protected]>wrote:

> Is it getting stuck at "PepXMLViewer.cgi"...
>
> I am not sure if it will help... do you have "tmp" folder under "C:" drive
> ?
>
> On Mon, Jul 30, 2012 at 7:33 PM, zeyu sun <[email protected]> wrote:
>
>>
>> Dear TPP team:
>> I came across a error message that I really cannot explain why. Hopefully 
>> some of experts here can help me to decode the message. Really appreciated!
>> The data was collected from a LTQ-Orbitrap, the search was done by X!tandem 
>> within the TPP environment using the default setting 
>> "isb_default_input_kscore setting".
>>
>>
>> The search result was automatically converted to pep.xml and when I try to 
>> filter the results with peptideprophet I got error message like this:
>> _____________________________________________________________________________________________________________________________
>>
>>
>>  run_in c:/Inetpub/wwwroot/ISB/data/data/AIF/20120728/processed; 
>> c:\Inetpub\tpp-bin\xinteract  -Nbsa1_3con_filtered.pep.xml -p0.95 -l7 -O 
>> bsa1_3con.tandem.pep.xml
>>
>> ------------------------------
>>
>> c:\Inetpub\tpp-bin\xinteract (TPP v4.5 RAPTURE rev 2, Build 201202031108 
>> (MinGW))
>>
>> running: "C:/Inetpub/tpp-bin/InteractParser "bsa1_3con_filtered.pep.xml" 
>> "bsa1_3con.tandem.pep.xml" -L"7""
>>  file 1: bsa1_3con.tandem.pep.xml
>>  processed altogether 4615 results
>>
>>
>>  results written to file 
>> c:/Inetpub/wwwroot/ISB/data/data/AIF/20120728/processed/bsa1_3con_filtered.pep.xml
>>
>>  direct your browser to 
>> http://localhost/ISB/data/data/AIF/20120728/processed/bsa1_3con_filtered.pep.shtml
>>
>>
>>
>> command completed in 1 sec
>>
>> running: "C:/Inetpub/tpp-bin/DatabaseParser "bsa1_3con_filtered.pep.xml""
>> command completed in 1 sec
>>
>> running: "C:/Inetpub/tpp-bin/RefreshParser "bsa1_3con_filtered.pep.xml" 
>> "c:/Inetpub/wwwroot/ISB/data/dbase/BOVIN_DECOY.fasta""
>>
>>
>> ------------------------------
>> * ...skipping 23 lines of output... *
>> ------------------------------
>>
>> WARNING: Mixture model quality test failed for charge (3+).
>>   model complete after 25 iterations
>> command completed in 1 sec
>>
>> running: "C:/Inetpub/tpp-bin/ProphetModels.pl -i bsa1_3con_filtered.pep.xml"
>> Analyzing bsa1_3con_filtered.pep.xml ...
>> Parsing search results 
>> "c:/Inetpub/wwwroot/ISB/data/data/AIF/20120728/processed/bsa1_3con (X! 
>> Tandem (k-score))"...
>>   => Total of 0 hits.
>>   => Total of 0 decoy hits.
>>   => Total of 0 excluded hits.
>> command completed in 0 sec
>>
>> running: "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I 
>> c:/Inetpub/wwwroot/ISB/data/data/AIF/20120728/processed/bsa1_3con_filtered.pep.xml"
>>
>> command "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I 
>> c:/Inetpub/wwwroot/ISB/data/data/AIF/20120728/processed/bsa1_3con_filtered.pep.xml"
>>  failed: Unknown error
>>
>> command "c:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I 
>> c:/Inetpub/wwwroot/ISB/data/data/AIF/20120728/processed/bsa1_3con_filtered.pep.xml"
>>  exited with non-zero exit code: 255
>> QUIT - the job is incomplete
>>
>> command "c:\Inetpub\tpp-bin\xinteract -Nbsa1_3con_filtered.pep.xml -p0.95 
>> -l7 -O bsa1_3con.tandem.pep.xml" failed: Unknown error
>>
>> *Command FAILED
>> __________________________________________________________
>>
>> thank you so much in advance!
>>
>> sunsun
>> *
>>
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