You may also want to check out DBToolkit: 
http://code.google.com/p/dbtoolkit.

Regards,
Andrew.

On Monday, July 30, 2012 8:59:35 PM UTC-4, sunsun wrote:
>
> thank you! actually I found another nice program the 
> ProteinDigestionSimulator, 
> http://omics.pnl.gov/software/ProteinDigestionSimulator.php. Both should 
> work! 
> sunsun
>
> On Mon, Jul 30, 2012 at 10:46 PM, Amit Yadav 
>
>> Hi
>> You can try digestdb.exe (comes with TPP)
>> Regards,
>>
>> *Amit Kumar Yadav *
>> Senior Research Fellow (SRF-CSIR)
>> IGIB, New Delhi (India)
>>
>> *MassWiz Web server* <http://masswiz.igib.res.in>
>> * <http://masswiz.igib.res.in>**MassWiz sourceforge 
>> project*<https://sourceforge.net/projects/masswiz>
>> * 
>> <https://sourceforge.net/projects/masswiz>**MassWiki*<https://sourceforge.net/apps/mediawiki/masswiz/index.php?title=MassWiki>
>>
>>
>>
>> On Mon, Jul 30, 2012 at 7:41 PM, zeyu sun
>>
>>> Hi,
>>> I'm wondering if any one here know how to predict all tryptic peptides 
>>> from the whole E.coli proteome. There are tools like PeptideCutter for 
>>> proteolytic prediction, but it can only cut one protein at each time. Any 
>>> tools around that can take the whole FASTA database and output a list of 
>>> tryptic peptides along with their masses? 
>>> thank you very much!
>>>
>>> sunsun
>>>
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>
>

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