Sounds like a pretty easy and worthwhile feature to add. Maybe you can
convince one of the ProteoWizard folks to add it.



In the mean time you could create one set of mzMLs with deisotoping for
searching, and then swap in a second set of mzMLs without to execute Libra.



Eric





*From:* [email protected] [mailto:
[email protected]] *On Behalf Of *Gautam Saxena
*Sent:* Friday, January 18, 2013 7:28 PM
*To:* [email protected]
*Subject:* [spctools-discuss] deisotoping in msconvert affects iTRAQ
reporter ion labels; can deisotoping only occur for >= 140 m/z



Deisotoping (in the msconvert option) significantly improves search results
for iTRAQ data. However, it also has very bad affects on the reporter ions.
Is there a way to get msconvert to only do deisotoping for those spectra in
which the m/z >= 140 (ie above the reporter ion levels)? Is this something
that can be customized? If not, is there a good workaround to this problem
-- eg running msconvert for ms2 below 140 (no desitoping) and again for
above 140 (with deisotoping) and somehow merging the mzXML files?

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