Dear ProteoWizard, I know msconvert is open source, so if we had the skills and expertise, we could make what appears to be an easy change, but therein lies the problem. Given our lack of expertise here, is there a proper way to request this feature enhancement so that it can be implemented "correctly"?
On Saturday, 19 January 2013 01:44:01 UTC-5, Eric Deutsch wrote: > > Sounds like a pretty easy and worthwhile feature to add. Maybe you can > convince one of the ProteoWizard folks to add it. > > > > In the mean time you could create one set of mzMLs with deisotoping for > searching, and then swap in a second set of mzMLs without to execute Libra. > > > > Eric > > > > > > *From:* [email protected] <javascript:> [mailto: > [email protected] <javascript:>] *On Behalf Of *Gautam Saxena > *Sent:* Friday, January 18, 2013 7:28 PM > *To:* [email protected] <javascript:> > *Subject:* [spctools-discuss] deisotoping in msconvert affects iTRAQ > reporter ion labels; can deisotoping only occur for >= 140 m/z > > > > Deisotoping (in the msconvert option) significantly improves search > results for iTRAQ data. However, it also has very bad affects on the > reporter ions. Is there a way to get msconvert to only do deisotoping for > those spectra in which the m/z >= 140 (ie above the reporter ion levels)? > Is this something that can be customized? If not, is there a good > workaround to this problem -- eg running msconvert for ms2 below 140 (no > desitoping) and again for above 140 (with deisotoping) and somehow merging > the mzXML files? > > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To view this discussion on the web visit > https://groups.google.com/d/msg/spctools-discuss/-/ka_LbRhkNXMJ. > To post to this group, send email to [email protected]<javascript:> > . > To unsubscribe from this group, send email to > [email protected] <javascript:>. > For more options, visit this group at > http://groups.google.com/group/spctools-discuss?hl=en. > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To view this discussion on the web visit https://groups.google.com/d/msg/spctools-discuss/-/4tyB8Gx-J2wJ. To post to this group, send email to [email protected]. To unsubscribe from this group, send email to [email protected]. For more options, visit this group at http://groups.google.com/group/spctools-discuss?hl=en.
