One possible solution is to keep everything in the C: drive, and relocate 
your data drive elsewhere using a hardlink or symlink. I have been using 
this with some success in windows 7 and windows 8. I moved my data folder 
to D:/TPP_MS_DATA/ and then I ran an elevated command prompt 
(Administrator) and then ran the command: 

mklink /j C:/Inetpub/wwwroot/data/ to D:/TPP_MS_DATA/data

Everything works as expected, all your data is stored somewhere else, and 
you don't have to worry about pathing issues, as even the operating 
system believes that the location of that directory is in 
C:/Inetpub/wwwroot/data/ instead of somewhere else on another drive.

Hope this helps!

Cheers,

David


On Friday, November 2, 2012 10:35:34 AM UTC-5, [email protected] wrote:
>
> Dear All,
>
> This is a "favorite" problem among TPP users, and we thought we had solved 
> it once and for all. But with the new TPP version (4.6.0 and 4.6.1) there 
> are issues with some of the tools when working with data on another drive 
> (G: in our case). I have changed the WEBSERVER_ROOT environment variable, 
> the tpp_gui.pl and of course the httpd.conf to point at G:, and keeping 
> the C:\Inetpub\tpp-bin\ directory. X!Tandem works, but not PeptideProphet 
> or the viewers.
>
> Below is our httpd.conf settings and the output from PeptideProphet.
>
> Changing everything back to C: makes everything work again, so the TPP 
> installation itself is OK I think. 
>
> Any ideas?
>
>
> Magnus
>
>
> httpd.conf:
>
> #
> # Begin settings for the Trans Proteomic Pipeline
> #
>
> # Add 24-hour timeout
> Timeout 86400
>
> Alias /tpp-bin "G:/Inetpub/tpp-bin"
> <Directory "G:/Inetpub/tpp-bin">
>     Options Indexes MultiViews ExecCGI
>     AllowOverride None
>     Order allow,deny
>     Allow from all
>
>     AddHandler cgi-script .cgi .pl
>     ScriptInterpreterSource Registry
>
>     PassEnv WEBSERVER_ROOT
>     PassEnv WEBSERVER_TMP
> </Directory>
>
> Alias /ISB "G:/Inetpub/wwwroot/ISB"
>
> Alias /schema "G:/Inetpub/wwwroot/schema"
>
> <Directory "G:/Inetpub/wwwroot">
>     Options Indexes MultiViews Includes
>     AllowOverride None
>     Order allow,deny
>     Allow from all
>
>     AddType text/html .shtml
>     AddHandler server-parsed .shtml
>
>     PassEnv WEBSERVER_ROOT
>     PassEnv WEBSERVER_TMP
> </Directory>
> #
> # End settings for the Trans Proteomic Pipeline
> #
>
>
> output from PeptideProphet:
>
> g:\Inetpub\tpp-bin\xinteract (TPP v4.6 OCCUPY rev 1, Build 201209261035 
> (MinGW)) running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" 
> "121009.LC1.IT1.EC.S0XXXX.Ecoli_1-B,1_01_3621.tandem.pep.xml" -L"7"" file 
> 1: 121009.LC1.IT1.EC.S0XXXX.Ecoli_1-B,1_01_3621.tandem.pep.xml processed 
> altogether 8922 results results written to file 
> g:/Inetpub/wwwroot/ISB/data/interact.pep.xml direct your browser to 
> http://localhost/ISB/data/interact.pep.shtml command completed in 2 sec 
> running: "C:/Inetpub/tpp-bin/DatabaseParser "interact.pep.xml"" command 
> completed in 0 sec running: "C:/Inetpub/tpp-bin/RefreshParser 
> "interact.pep.xml" "g:/Inetpub/wwwroot/ISB/data/dbase/18.E_coli_K12.fasta"" 
> - Searching the tree... - Linking duplicate entries... - Printing 
> results... - Building Commentz-Walter keyword tree...command completed in 1 
> sec running: "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml" 
> MINPROB=0.05" (X! Tandem (k-score)) init with X! Tandem (k-score) trypsin 
> MS Instrument info: Manufacturer: Bruker Daltonics, Model: esquire, 
> Ionization: ESI, Analyzer: Quadrupole Ion Trap, Detector: MS:1000253 
> PeptideProphet (TPP v4.6 OCCUPY rev 1, Build 201209261035 (MinGW)) 
> AKeller@ISB read in 31 1+, 4238 2+, 4472 3+, 0 4+, 0 5+, 0 6+, and 0 7+ 
> spectra. Initialising statistical models ... negmean = -1.1254 negmean = 
> -1.74678 negmean = -1.51536 negmean = -0.2 negmean = -0.2 negmean = -0.2 
> negmean = -0.2 Iterations: .........10.........20......... model complete 
> after 30 iterations command completed in 1 sec running: 
> "C:/Inetpub/tpp-bin/ProphetModels.pl -i interact.pep.xml" Analyzing 
> interact.pep.xml ... Parsing search results 
> "g:/Inetpub/wwwroot/ISB/data/121009.LC1.IT1.EC.S0XXXX.Ecoli_1-B,1_01_3621 
> (X! Tandem (k-score))"... => Total of 1623 hits. => Total of 0 decoy hits. 
> => Total of 0 excluded hits. command completed in 1 sec running: 
> "G:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I 
> g:/Inetpub/wwwroot/ISB/data/interact.pep.xml" This application has 
> requested the Runtime to terminate it in an unusual way. Please contact the 
> application's support team for more information. command 
> "G:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I 
> g:/Inetpub/wwwroot/ISB/data/interact.pep.xml" failed: Unknown error command 
> "G:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I 
> g:/Inetpub/wwwroot/ISB/data/interact.pep.xml" exited with non-zero exit 
> code: 255 QUIT - the job is incomplete command 
> "g:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -O 
> 121009.LC1.IT1.EC.S0XXXX.Ecoli_1-B,1_01_3621.tandem.pep.xml" failed: 
> Unknown error 
>
>

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