This actually will not work, as by default the tpp_gui.pl script wont 
follow symlinks correctly. Ignore previous post.

On Monday, January 28, 2013 2:44:24 PM UTC-6, David wrote:
>
> One possible solution is to keep everything in the C: drive, and relocate 
> your data drive elsewhere using a hardlink or symlink. I have been using 
> this with some success in windows 7 and windows 8. I moved my data folder 
> to D:/TPP_MS_DATA/ and then I ran an elevated command prompt 
> (Administrator) and then ran the command: 
>
> mklink /j C:/Inetpub/wwwroot/data/ to D:/TPP_MS_DATA/data
>
> Everything works as expected, all your data is stored somewhere else, and 
> you don't have to worry about pathing issues, as even the operating 
> system believes that the location of that directory is in 
> C:/Inetpub/wwwroot/data/ instead of somewhere else on another drive.
>
> Hope this helps!
>
> Cheers,
>
> David
>
>
> On Friday, November 2, 2012 10:35:34 AM UTC-5, [email protected] wrote:
>>
>> Dear All,
>>
>> This is a "favorite" problem among TPP users, and we thought we had 
>> solved it once and for all. But with the new TPP version (4.6.0 and 4.6.1) 
>> there are issues with some of the tools when working with data on another 
>> drive (G: in our case). I have changed the WEBSERVER_ROOT environment 
>> variable, the tpp_gui.pl and of course the httpd.conf to point at G:, 
>> and keeping the C:\Inetpub\tpp-bin\ directory. X!Tandem works, but not 
>> PeptideProphet or the viewers.
>>
>> Below is our httpd.conf settings and the output from PeptideProphet.
>>
>> Changing everything back to C: makes everything work again, so the TPP 
>> installation itself is OK I think. 
>>
>> Any ideas?
>>
>>
>> Magnus
>>
>>
>> httpd.conf:
>>
>> #
>> # Begin settings for the Trans Proteomic Pipeline
>> #
>>
>> # Add 24-hour timeout
>> Timeout 86400
>>
>> Alias /tpp-bin "G:/Inetpub/tpp-bin"
>> <Directory "G:/Inetpub/tpp-bin">
>>     Options Indexes MultiViews ExecCGI
>>     AllowOverride None
>>     Order allow,deny
>>     Allow from all
>>
>>     AddHandler cgi-script .cgi .pl
>>     ScriptInterpreterSource Registry
>>
>>     PassEnv WEBSERVER_ROOT
>>     PassEnv WEBSERVER_TMP
>> </Directory>
>>
>> Alias /ISB "G:/Inetpub/wwwroot/ISB"
>>
>> Alias /schema "G:/Inetpub/wwwroot/schema"
>>
>> <Directory "G:/Inetpub/wwwroot">
>>     Options Indexes MultiViews Includes
>>     AllowOverride None
>>     Order allow,deny
>>     Allow from all
>>
>>     AddType text/html .shtml
>>     AddHandler server-parsed .shtml
>>
>>     PassEnv WEBSERVER_ROOT
>>     PassEnv WEBSERVER_TMP
>> </Directory>
>> #
>> # End settings for the Trans Proteomic Pipeline
>> #
>>
>>
>> output from PeptideProphet:
>>
>> g:\Inetpub\tpp-bin\xinteract (TPP v4.6 OCCUPY rev 1, Build 201209261035 
>> (MinGW)) running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml" 
>> "121009.LC1.IT1.EC.S0XXXX.Ecoli_1-B,1_01_3621.tandem.pep.xml" -L"7"" file 
>> 1: 121009.LC1.IT1.EC.S0XXXX.Ecoli_1-B,1_01_3621.tandem.pep.xml processed 
>> altogether 8922 results results written to file 
>> g:/Inetpub/wwwroot/ISB/data/interact.pep.xml direct your browser to 
>> http://localhost/ISB/data/interact.pep.shtml command completed in 2 sec 
>> running: "C:/Inetpub/tpp-bin/DatabaseParser "interact.pep.xml"" command 
>> completed in 0 sec running: "C:/Inetpub/tpp-bin/RefreshParser 
>> "interact.pep.xml" "g:/Inetpub/wwwroot/ISB/data/dbase/18.E_coli_K12.fasta"" 
>> - Searching the tree... - Linking duplicate entries... - Printing 
>> results... - Building Commentz-Walter keyword tree...command completed in 1 
>> sec running: "C:/Inetpub/tpp-bin/PeptideProphetParser "interact.pep.xml" 
>> MINPROB=0.05" (X! Tandem (k-score)) init with X! Tandem (k-score) trypsin 
>> MS Instrument info: Manufacturer: Bruker Daltonics, Model: esquire, 
>> Ionization: ESI, Analyzer: Quadrupole Ion Trap, Detector: MS:1000253 
>> PeptideProphet (TPP v4.6 OCCUPY rev 1, Build 201209261035 (MinGW)) 
>> AKeller@ISB read in 31 1+, 4238 2+, 4472 3+, 0 4+, 0 5+, 0 6+, and 0 7+ 
>> spectra. Initialising statistical models ... negmean = -1.1254 negmean = 
>> -1.74678 negmean = -1.51536 negmean = -0.2 negmean = -0.2 negmean = -0.2 
>> negmean = -0.2 Iterations: .........10.........20......... model complete 
>> after 30 iterations command completed in 1 sec running: 
>> "C:/Inetpub/tpp-bin/ProphetModels.pl -i interact.pep.xml" Analyzing 
>> interact.pep.xml ... Parsing search results 
>> "g:/Inetpub/wwwroot/ISB/data/121009.LC1.IT1.EC.S0XXXX.Ecoli_1-B,1_01_3621 
>> (X! Tandem (k-score))"... => Total of 1623 hits. => Total of 0 decoy hits. 
>> => Total of 0 excluded hits. command completed in 1 sec running: 
>> "G:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I 
>> g:/Inetpub/wwwroot/ISB/data/interact.pep.xml" This application has 
>> requested the Runtime to terminate it in an unusual way. Please contact the 
>> application's support team for more information. command 
>> "G:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I 
>> g:/Inetpub/wwwroot/ISB/data/interact.pep.xml" failed: Unknown error command 
>> "G:/Inetpub/wwwroot/../tpp-bin/PepXMLViewer.cgi -I 
>> g:/Inetpub/wwwroot/ISB/data/interact.pep.xml" exited with non-zero exit 
>> code: 255 QUIT - the job is incomplete command 
>> "g:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -O 
>> 121009.LC1.IT1.EC.S0XXXX.Ecoli_1-B,1_01_3621.tandem.pep.xml" failed: 
>> Unknown error 
>>
>>

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