Hi Lqd, great, I’m glad it works. I now vaguely recall that TPP 4.5 had a
problem where .Net was not automatically installed properly. I think this
has been fixed or documented in later versions. Do upgrade if you can!



Regards,

Eric





*From:* [email protected] [mailto:[email protected]]
*Sent:* Sunday, February 03, 2013 9:21 PM
*To:* Eric Deutsch
*Subject:* Re: Problem with data convert by TPP



Hi! Thank you very much.

I just downloaded and setup .net framework 4.0. It seems work. .Net
framework 4.0 seems required by MSConvert.

Your suggestion and forwarding my question are still very important for me.

Best Regards!

Lqd

Sent from my iPad


On 2013-2-4, at 12:52, "Eric Deutsch" <[email protected]>
wrote:

Hi lqd, I am not currently familiar with the state of converting Waters
.raw/ data with the msconvert bundled with TPP 4.5, but I am forwarding to
the spctools-discuss list where someone will more likely know the answer.
Maybe an additional download of libraries from Waters is needed. However, I
would like to suggest you download and install TPP 4.6.1 instead:



http://sourceforge.net/projects/sashimi/files/Trans-Proteomic%20Pipeline%20%28TPP%29/



4.6.2 will be released very soon, as well. Version 4.5 was released exactly
a year ago, so a newer version is recommended.



Regards,

Eric





*From:* lqd [mailto:[email protected]]
*Sent:* Sunday, February 03, 2013 5:11 PM
*To:* edeutsch
*Subject:* Problem with data convert by TPP





Dear Sir,

    I need to convert my Waters LCMS data (format as data in .RAW
directory).

    I download Trans-Proteomic Pipeline v4_5 and setup it in my PC. But it
does not work at all.

    I tried log in as guest or log in using my own ID, I tried convert from
.RAW to mzXML or to mzML, but all failed. Nothing was output.

    The output content is the following:

   - Output Files


   - c:/Inetpub/wwwroot/ISB/data/Female6_pos.mzML (*file not found:* not
      created or deleted?)
      - c:/Inetpub/wwwroot/ISB/data/Female7_neg.mzML (*file not found:* not
      created or deleted?)



  Command 1 [Mon Feb 4 08:51:10 2013]

   [ Show / Hide ]

*EXECUTING:** c:\Inetpub\tpp-bin\msconvert
c:/Inetpub/wwwroot/ISB/data/Female6_pos.raw -v --mzML -o
c:/Inetpub/wwwroot/ISB/data *

*Command Successful*
RETURN CODE:0



  Command 2 [Mon Feb 4 08:51:10 2013]

   [ Show / Hide ]

*EXECUTING:** c:\Inetpub\tpp-bin\msconvert
c:/Inetpub/wwwroot/ISB/data/Female7_neg.raw -v --mzML -o
c:/Inetpub/wwwroot/ISB/data *

*Command Successful*
RETURN CODE:0



 All commands finished at *Mon Feb 4 08:51:10 2013*


I would appreciate if you could give me some help.

Best Regards

Qiande Liang

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