Hi,

I was wondering if anyone could help me or offer any suggestions towards
issues I have been having with TPP software. I have been trying to build a
spectral library from data gathered on a Q-Tof Mass Spectrometer from crude
synthesised phosphorylated peptides. Although I am able to see the peptides
when analysing the data using PEAKS softwar, they do not appear in the
final library built using TPP when I import it into Skyline (MacCoss lab).
I don't understand if this is a problem with TPP or Skyline or the fact
that I am trying to find phosphorylated peptides and am possibly missing an
extra step in the TPP pipeline.

I would really appreciate any help or advice with this issue!

Thanks,

Claire Tonry (MRes)
Conway Institute of Biomedical and Biomolecular Science

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