Hi, it sounds like you have not enabled the consensus option, and thus created a raw library. See:
http://tools.proteomecenter.org/wiki/index.php?title=Software:SpectraST#Creating_Consensus_Libraries Regards, Eric *From:* [email protected] [mailto: [email protected]] *On Behalf Of *S.K.V. Adhin *Sent:* Monday, June 10, 2013 2:10 AM *To:* [email protected] *Subject:* [spctools-discuss] Removing redundant spectra Recently I made a large spectral library made 30 raw files from the Q-Exactive. After the mzXML conversion with msconvert I did a database search with X!Tandem (using TPP) and retrieved 30 pep.xml files. Subsequently I merged the 30 files with Peptideprophet into one interact.pep.xml file.This file I used to build a spectral library with SpectraST (also using the TPP). This resulted in a huge spectral library with a filesize of 5GB. I was wondering why this was such a huge file so I inspected the file with spectral library viewer in Skyline. I noticed that there many duplicate entries in the spectral library. I know that one peptide can be assigned to different spectra so peptides in the library does not have to be unique. But I found out that the spectral library contained the same peptide assigned to the same spectra multiple (between 10 and 25) times. Is there a particular reason why the spectral library have so many exactly the same entries and is there a possibility to remove them, so that at least every spectra in the library is unique? -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at http://groups.google.com/group/spctools-discuss?hl=en. For more options, visit https://groups.google.com/groups/opt_out. -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at http://groups.google.com/group/spctools-discuss?hl=en. For more options, visit https://groups.google.com/groups/opt_out.
