Hi Jason,

Not sure but this might help
https://groups.google.com/forum/#!topic/spctools-discuss/QSUZQ3ViRoQ

Thanks


On Wed, Apr 2, 2014 at 5:55 AM, jason cao <[email protected]> wrote:

>           I am  struggling to make TPP 4.7 working in Windows 8.
>
>          I am follow the description  of  " Quick Start to data analysis
> using the TPP"(
> http://tools.proteomecenter.org/wiki/index.php?title=TPP_Tutorial).Step1
> - step5   is  Ok! Mistakes is happen in step6 ---Validation of
> Peptide-Spectrum assignments with PeptideProphet.
>
>         When i  *Run XInteract.*The commends finished immediately,and
> showed *Command FAILED    *RETURN CODE:256.
>
>         Next is the advice and the context of failed commends :
>
>      (I have set the environment variable to my computer,the outcme still
> wrong.Plz help me ,thank you ! )
>
>       running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml"
> "OR20080317_S_SILAC-LH_1-1_01.tandem.pep.xml"
> "OR20080320_S_SILAC-LH_1-1_11.tandem.pep.xml" -L"7""
>
> *<PRE> Environment variable WEBSERVER_ROOT does not exist.
>
>  For Windows users, you can set this environment variable
>  through the Advanced tab under System Properties when you
>  right-mouse-click on your My Computer icon.
>
>  Set this environment variable to your webserver's document
>  root directory such as c:\inetpub\wwwroot for IIS or
>  c:\website\htdocs or WebSite Pro.
>
>  Exiting.
> command completed in 1.39643e+009 sec
>
> running: "C:/Inetpub/tpp-bin/DatabaseParser "interact.pep.xml""
> pipe "C:/Inetpub/tpp-bin/DatabaseParser "interact.pep.xml"" failed: No error
> error, could not execute: C:/Inetpub/tpp-bin/DatabaseParser "interact.pep.xml"
>
> command "c:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -OA 
> OR20080317_S_SILAC-LH_1-1_01.tandem.pep.xml 
> OR20080320_S_SILAC-LH_1-1_11.tandem.pep.xml" failed: Operation not permitted*
>
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