Jason,

Try this:

Right Click "My Computer" -> Properties -> Advanced System Settings ->
Advanced-> Environment Variables

Here add a *System* variable

WEBSERVER_ROOT c:\Inetpub\wwwroot

Also here make sure that *Path* variable includes c:\Inetpub\tpp-bin and
also your Perl installation usually c:\Perl\bin

You may need to reboot or restart Apache in order for the setting to take
effect.

This is meant to be set by the installer but is not always possible on all
systems.  You can see the environment variables set in Apache by going to:
http://localhost/tpp-bin/check_env.pl


Let us know if you need further assistance.


-David





On Wed, Apr 2, 2014 at 9:22 AM, Vishal Swaroop <[email protected]> wrote:

> Hi Jason,
>
> Not sure but this might help
> https://groups.google.com/forum/#!topic/spctools-discuss/QSUZQ3ViRoQ
>
> Thanks
>
>
> On Wed, Apr 2, 2014 at 5:55 AM, jason cao <[email protected]> wrote:
>
>>           I am  struggling to make TPP 4.7 working in Windows 8.
>>
>>          I am follow the description  of  " Quick Start to data analysis
>> using the TPP"(
>> http://tools.proteomecenter.org/wiki/index.php?title=TPP_Tutorial).Step1
>> - step5   is  Ok! Mistakes is happen in step6 ---Validation of
>> Peptide-Spectrum assignments with PeptideProphet.
>>
>>         When i  *Run XInteract.*The commends finished immediately,and
>> showed *Command FAILED    *RETURN CODE:256.
>>
>>         Next is the advice and the context of failed commends :
>>
>>      (I have set the environment variable to my computer,the outcme
>> still wrong.Plz help me ,thank you ! )
>>
>>       running: "C:/Inetpub/tpp-bin/InteractParser "interact.pep.xml"
>> "OR20080317_S_SILAC-LH_1-1_01.tandem.pep.xml"
>> "OR20080320_S_SILAC-LH_1-1_11.tandem.pep.xml" -L"7""
>>
>> *<PRE> Environment variable WEBSERVER_ROOT does not exist.
>>
>>  For Windows users, you can set this environment variable
>>  through the Advanced tab under System Properties when you
>>  right-mouse-click on your My Computer icon.
>>
>>  Set this environment variable to your webserver's document
>>  root directory such as c:\inetpub\wwwroot for IIS or
>>  c:\website\htdocs or WebSite Pro.
>>
>>  Exiting.
>> command completed in 1.39643e+009 sec
>>
>> running: "C:/Inetpub/tpp-bin/DatabaseParser "interact.pep.xml""
>> pipe "C:/Inetpub/tpp-bin/DatabaseParser "interact.pep.xml"" failed: No error
>> error, could not execute: C:/Inetpub/tpp-bin/DatabaseParser 
>> "interact.pep.xml"
>>
>> command "c:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -OA 
>> OR20080317_S_SILAC-LH_1-1_01.tandem.pep.xml 
>> OR20080320_S_SILAC-LH_1-1_11.tandem.pep.xml" failed: Operation not permitted*
>>
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