Thank you for your help David,

I ended up fixing it by doing a clean install of ActivePerl 5.18.2 and TPP 
4.7.0. I also changed the Admin address for Apache during the installation, 
just in case it made a difference. Either way, after the clean install 
X!Tandem seems to work fine. I have a feeling it had something to do with 
conflicting versions of ActivePerl on this computer.

-Will

On Saturday, April 19, 2014 12:34:45 PM UTC-4, David Shteynberg wrote:
>
> If the same command worked on the commandline and not through Petunia then 
> the problem is not with the parameters but maybe with the system 
> environment.  Can you tell us more about the system you are running this 
> on?  The paths looks like windows and yet you attached params file contains 
> linux paths:
>
> <note type="input" label="list path, taxonomy information">
> /usr/local/tpp/data/parameters/taxonomy.xml</note>
> <note type="input" label="protein, taxon">protein_database</note>
>
>
> See if this works better:
>
>
> <note type="input" label="list path, taxonomy information">
> C:\Inetpub\wwwroot\ISB\data\parameters\taxonomy.xml</note>
> <note type="input" label="protein, taxon">protein_database</note>
>
>
> -David
>
>
> On Fri, Apr 18, 2014 at 12:32 PM, Will Fondrie <[email protected]<javascript:>
> > wrote:
>
>> Hi everyone,
>>
>> I'm now having trouble getting an X!Tandem search working through the 
>> Petunia GUI on my Windows machine. Whenever I try to start a search I 
>> receive this error:
>>
>> *EXECUTING: run_in c:/Inetpub/wwwroot/ISB/data/Will; 
>> c:\Inetpub\tpp-bin\tandem 
>> c:/Inetpub/wwwroot/ISB/data/Will/test.tandem.params *
>>
>> command "c:\Inetpub\tpp-bin\tandem 
>> c:/Inetpub/wwwroot/ISB/data/Will/test.tandem.params" failed: Unknown error
>>
>> *Command FAILED*
>> RETURN CODE:65280
>>
>> However, I have no problems doing the same search, with the same 
>> parameters file from the command line. Any suggestions about what may be 
>> going on?
>>
>> I've attached that parameter file that I'm using as well.
>>
>> Thank you,
>> Will
>>
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