Hi Joe,

Thanks for your reply. It probably is a file size, yes (mzml file size is 
~3gb). I'm using a virtualmachine (windows XP -32bt system) on an iMAC with 
8gb RAM and 2.70GHz


I have tried to do it with the MSConvert software (the GUI from 
Poteowizard) and there's no problem, then I can rename the file and run it 
in TPP with no issues, so I might do that instead of using the integrated 
mscovert. The failed mzml has ~1Gb whereas the good mzml is about ~3Gb.

Cheers
Javier



El jueves, 13 de noviembre de 2014 15:39:58 UTC+10, 
[email protected] escribió:
>
> Javier,
>
> Sounds like it might be a memory/file size issue.  First off I'd check the 
> last line of the file to see if it has the mzML end marker.  If it isn't 
> "</mzML>" then you know msconvert didnt' finish converting the original 
> file into mzML.  Also, how big is the original file that you are trying to 
> convert?  What is the size of your mzML file?  And is your computer a 
> 32-bit operating system? 
>
> -Joe
>
>
> On Wed, Nov 12, 2014 at 6:11 PM, Javier Sotillo Gallego <[email protected] 
> <javascript:>> wrote:
>
>> Hi all,
>>
>> I'm trying to convert wiff files to mzml files and despite the command is 
>> successful I encounter this error during the conversion:
>>
>> Error writing run 1 in "1.wiff":
>> bad allocation
>>
>> The converter saves a mzml file, but cannot open it with Pep3D to check 
>> the quality of the data.
>>
>> Any ideas?
>>
>> Thanks guys!!!!
>>
>> Here's a screenshot
>>
>>
>> <https://lh3.googleusercontent.com/-NpC3xhJLxfw/VGQSp6ljXcI/AAAAAAAAAJY/_amca9pPW7Y/s1600/Screen%2BShot%2B2014-11-13%2Bat%2B11.57.19%2BAM%2B%282%29.png>
>>
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>
>
>
> -- 
> Joe Slagel
> Institute for Systems Biology
> [email protected] <javascript:>
>
> 

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