Hi Sebastian,
It seems despite limiting the number of spectra, you still are running
low on memory when running Comet. I am guessing that the spectra are
high resolution and require a really small fragment_bin_tol? If this is
not the case, let me know. Otherwise, try setting spectrum_batch_size to
smaller values until the search can be conducted with the available
memory on your system. You can also save some memory by slightly
increasing your fragment_bin_tol, for example, from 0.02 to 0.03.
Cheers,
Mike
On 1/28/2015 9:19 AM, Sebastian Wiese wrote:
Hi all,
I'm currently running TPP v4.7 with Comet Version 2014.01 rev. 0
When running a .mzml file converted via Ms Convert from a Thermo
.RAW-file, I get the follwing error:
Comet version "2014.01 rev. 0"
Search start: 01/28/2015, 04:22:17 PM
- Input file: CUPro02488.mzML
- Load and process input spectra Error
- calloc(pdTempRawData[110704]). Error
- calloc(pdTmpFastXcorrData[93904]).
command "c:\Inetpub\tpp-bin\comet
-Pd:/TPP-Data/Data/Comet/comet.params.high-high-AGB
-Dd:/TPP-Data/Data/Sequences/HUMAN_DECOY.fasta CUPro02488.mzML"
failed: Operation not permitted
Calling comet in the command line causes the same erros. I already
limited the number of spectra searched at once as mentioned in a
previous comment regarding the Comet engine.
Other searches using Comet worked and I cannot see what is causing
this error.
i would aprreciate any help on this subject.
Yours,
Sebastian
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