Hi Mike, You are right, we are trying to analyze data from TMT-labelled samples. I will try to lower the number of spectra and the sampling bin.
Thanks for your help, I'll post if this solved the problem. Yours Sebastian Am Mittwoch, 28. Januar 2015 18:35:50 UTC+1 schrieb Michael Hoopmann: > > Hi Sebastian, > It seems despite limiting the number of spectra, you still are running low > on memory when running Comet. I am guessing that the spectra are high > resolution and require a really small fragment_bin_tol? If this is not the > case, let me know. Otherwise, try setting spectrum_batch_size to smaller > values until the search can be conducted with the available memory on your > system. You can also save some memory by slightly increasing your > fragment_bin_tol, for example, from 0.02 to 0.03. > Cheers, > Mike > > On 1/28/2015 9:19 AM, Sebastian Wiese wrote: > > Hi all, > > I'm currently running TPP v4.7 with Comet Version 2014.01 rev. 0 > > When running a .mzml file converted via Ms Convert from a Thermo > .RAW-file, I get the follwing error: > > Comet version "2014.01 rev. 0" > Search start: 01/28/2015, 04:22:17 PM > - Input file: CUPro02488.mzML > - Load and process input spectra Error > - calloc(pdTempRawData[110704]). Error > - calloc(pdTmpFastXcorrData[93904]). > > command "c:\Inetpub\tpp-bin\comet > -Pd:/TPP-Data/Data/Comet/comet.params.high-high-AGB > -Dd:/TPP-Data/Data/Sequences/HUMAN_DECOY.fasta CUPro02488.mzML" failed: > Operation not permitted > > Calling comet in the command line causes the same erros. I already limited > the number of spectra searched at once as mentioned in a previous comment > regarding the Comet engine. > > Other searches using Comet worked and I cannot see what is causing this > error. > > i would aprreciate any help on this subject. > > Yours, > Sebastian > -- > You received this message because you are subscribed to the Google Groups > "spctools-discuss" group. > To unsubscribe from this group and stop receiving emails from it, send an > email to [email protected] <javascript:>. > To post to this group, send email to [email protected] > <javascript:>. > Visit this group at http://groups.google.com/group/spctools-discuss. > For more options, visit https://groups.google.com/d/optout. > > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at http://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
