Hi all,

I am getting the following error when trying to run protein prophet:

*EXECUTING: c:\Inetpub\tpp-bin\ProteinProphet c:/Inetpub/wwwroot/ISB/data/Fusion/141205_MS2_CID_ETD_Meyer_J_UCSD/MGplus/mgpl.dbupdate.quant.interact.ptm.pep.xml c:/Inetpub/wwwroot/ISB/data/Fusion/141205_MS2_CID_ETD_Meyer_J_UCSD/MGplus/20150203.testprotein3.interact.prot.xml IPROPHET XPRESS EXCLUDE_ZEROS PROTLEN PROTMW *

ProteinProphet (C++) by Insilicos LLC and LabKey Software, after the original 
Perl by A. Keller (TPP v4.8.0 PHILAE, Build 201411201551-6764 (mingw-i686))
 (xml input) (no FPKM) (XPRESS) (report Protein Length) (report Protein Mol 
Weights) (using degen pep info) (exclude zero prob entries)
. . . reading in 
c:/Inetpub/wwwroot/ISB/data/Fusion/141205_MS2_CID_ETD_Meyer_J_UCSD/MGplus/mgpl.dbupdate.quant.interact.ptm.pep.xml.
 . .
. . . read in 0 1+, 6914 2+, 12598 3+, 8315 4+, 1131 5+, 77 6+, 0 7+ spectra 
with min prob 0.05
DNS server not authoritative for zone.

command "C:/Inetpub/tpp-bin/batchcoverage "f:/MSGFPlus.20140716/database/110712_human.cc.fasta" 
"c:/Inetpub/wwwroot/ISB/data/Fusion/141205_MS2_CID_ETD_Meyer_J_UCSD/MGplus/20150203.testprotein3.interact.prot.xml.covinfo"
 
"c:/Inetpub/wwwroot/ISB/data/Fusion/141205_MS2_CID_ETD_Meyer_J_UCSD/MGplus/20150203.testprotein3.interact.prot.xml.cov""
 failed: Operation not permitted
command "C:/Inetpub/tpp-bin/batchcoverage "f:/MSGFPlus.20140716/database/110712_human.cc.fasta" 
"c:/Inetpub/wwwroot/ISB/data/Fusion/141205_MS2_CID_ETD_Meyer_J_UCSD/MGplus/20150203.testprotein3.interact.prot.xml.covinfo"
 
"c:/Inetpub/wwwroot/ISB/data/Fusion/141205_MS2_CID_ETD_Meyer_J_UCSD/MGplus/20150203.testprotein3.interact.prot.xml.cov""
 failed with exit code 1

*Command FAILED*
RETURN CODE:256

Any help would be appreciated.

Best,
Jesse

--
Jesse Meyer
Graduate Student
Department of Chemistry and Biochemistry
University of California, San Diego
La Jolla, CA 92092



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