Hi Alves,

I solved this problem by re-running the TPP installer. No uninstall needed. There must have been some problem with the webserver settings. Let me know if that solves it for you as well.

Best,
Jesse


On 2/4/2015 8:49 AM, Alves wrote:
Hi Jesse,
I'm getting exactly same error right now, but with the tutorial data. So, it seems that there is something related to pipeline or ProtProphet instead of the data. I did try rerun all the tutorial exactly as they teach 3-4 times, but it didn't work.
Don't know what it would be.

Em terça-feira, 3 de fevereiro de 2015 20:49:14 UTC-2, Jesse escreveu:

    Hi all,

    I am getting the following error when trying to run protein prophet:

    *EXECUTING: c:\Inetpub\tpp-bin\ProteinProphet
    
c:/Inetpub/wwwroot/ISB/data/Fusion/141205_MS2_CID_ETD_Meyer_J_UCSD/MGplus/mgpl.dbupdate.quant.interact.ptm.pep.xml
    
c:/Inetpub/wwwroot/ISB/data/Fusion/141205_MS2_CID_ETD_Meyer_J_UCSD/MGplus/20150203.testprotein3.interact.prot.xml
    IPROPHET XPRESS EXCLUDE_ZEROS PROTLEN PROTMW *

    ProteinProphet (C++) by Insilicos LLC and LabKey Software, after the 
original Perl by A. Keller (TPP v4.8.0 PHILAE, Build 201411201551-6764 
(mingw-i686))
      (xml input) (no FPKM) (XPRESS) (report Protein Length) (report Protein 
Mol Weights) (using degen pep info) (exclude zero prob entries)
    . . . reading in 
c:/Inetpub/wwwroot/ISB/data/Fusion/141205_MS2_CID_ETD_Meyer_J_UCSD/MGplus/mgpl.dbupdate.quant.interact.ptm.pep.xml.
 . .
    . . . read in 0 1+, 6914 2+, 12598 3+, 8315 4+, 1131 5+, 77 6+, 0 7+ 
spectra with min prob 0.05
    DNS server not authoritative for zone.

    command "C:/Inetpub/tpp-bin/batchcoverage "f:/MSGFPlus.20140716/database/110712_human.cc.fasta" 
"c:/Inetpub/wwwroot/ISB/data/Fusion/141205_MS2_CID_ETD_Meyer_J_UCSD/MGplus/20150203.testprotein3.interact.prot.xml.covinfo"
 
"c:/Inetpub/wwwroot/ISB/data/Fusion/141205_MS2_CID_ETD_Meyer_J_UCSD/MGplus/20150203.testprotein3.interact.prot.xml.cov""
 failed: Operation not permitted
    command "C:/Inetpub/tpp-bin/batchcoverage "f:/MSGFPlus.20140716/database/110712_human.cc.fasta" 
"c:/Inetpub/wwwroot/ISB/data/Fusion/141205_MS2_CID_ETD_Meyer_J_UCSD/MGplus/20150203.testprotein3.interact.prot.xml.covinfo"
 
"c:/Inetpub/wwwroot/ISB/data/Fusion/141205_MS2_CID_ETD_Meyer_J_UCSD/MGplus/20150203.testprotein3.interact.prot.xml.cov""
 failed with exit code 1

    *Command FAILED*
    RETURN CODE:256

    Any help would be appreciated.

    Best,
    Jesse

-- Jesse Meyer
    Graduate Student
    Department of Chemistry and Biochemistry
    University of California, San Diego
    La Jolla, CA 92092



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Jesse Meyer
Graduate Student
Department of Chemistry and Biochemistry
University of California, San Diego
La Jolla, CA 92092



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