Hi,
I created a spectra library as follows, however there is error when I tried
to create an consensus library from the built library.
Can anyone identify the problem?
Thanks so much!
Regards,
Rachel
===========================================
Total number of spectra in library: 648544
Total number of distinct peptide ions in library: 145857
Total number of distinct stripped peptides in library: 98849
CHARGE +1: 0 ; +2: 365720 ; +3: 236872 ; +4: 45952 ; +5: 0 ;
>+5: 0
TERMINI Tryptic: 639576 ; Semi-tryptic: 8959 ; Non-tryptic: 9
PROBABILITY >0.9999: 385116 ; 0.999-0.9999: 102413 ; 0.99-0.999:
77182 ; 0.9-0.99: 56492 ; <0.9: 27341
NREPS 20+: 0 ; 10-19: 0 ; 4-9: 0 ; 2-3: 0 ; 1: 648544
MODIFICATIONS C,Carbamidomethyl: 126928 ; C,Pyro-carbamidomethyl: 8477
E,Glu->pyro-Glu: 3684
M,Oxidation: 62131
Q,Gln->pyro-Glu: 13812
n,Acetyl: 9797
Total Run Time = 5009 seconds.
SpectraST finished at Mon Feb 23 15:04:05 2015 without error.
SpectraST started at Mon Feb 23 15:04:05 2015.
Creating CONSENSUS library from "c:/inetpub/tpp-bin/Spectralib.splib"
Importing ions... ==== FATAL ERROR. Exiting immediately. ====
Error trace :
GENERAL: Corrupt .splib file from which to import entry.
===========================================
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