Why spectrast.exe can build library successfully, but not consensus spectra? Eric has suggested to download spectrast from http://sourceforge.net/projects/sashimi/files/Trans-Proteomic%20Pipeline%20%28TPP%29/TPP%20v0.0%20%28Development%29/ , but it does not work for me. I did not have a server to try TPP. So is there a way to fix the problem on windows?
Thank you! Rachel On Tuesday, February 24, 2015 at 10:08:53 AM UTC+8, Rachel wrote: > > Hi, > I created a spectra library as follows, however there is error when I > tried to create an consensus library from the built library. > Can anyone identify the problem? > Thanks so much! > Regards, > Rachel > =========================================== > Total number of spectra in library: 648544 > Total number of distinct peptide ions in library: 145857 > Total number of distinct stripped peptides in library: 98849 > > CHARGE +1: 0 ; +2: 365720 ; +3: 236872 ; +4: 45952 ; +5: 0 ; > >+5: 0 > TERMINI Tryptic: 639576 ; Semi-tryptic: 8959 ; Non-tryptic: 9 > PROBABILITY >0.9999: 385116 ; 0.999-0.9999: 102413 ; 0.99-0.999: > 77182 ; 0.9-0.99: 56492 ; <0.9: 27341 > NREPS 20+: 0 ; 10-19: 0 ; 4-9: 0 ; 2-3: 0 ; 1: 648544 > MODIFICATIONS C,Carbamidomethyl: 126928 ; C,Pyro-carbamidomethyl: 8477 > E,Glu->pyro-Glu: 3684 > M,Oxidation: 62131 > Q,Gln->pyro-Glu: 13812 > n,Acetyl: 9797 > > Total Run Time = 5009 seconds. > SpectraST finished at Mon Feb 23 15:04:05 2015 without error. > SpectraST started at Mon Feb 23 15:04:05 2015. > Creating CONSENSUS library from "c:/inetpub/tpp-bin/Spectralib.splib" > Importing ions... ==== FATAL ERROR. Exiting immediately. ==== > Error trace : > GENERAL: Corrupt .splib file from which to import entry. > =========================================== > > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at http://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
