Thank you for your suggestion!

在 2015年4月18日星期六 UTC+8下午11:37:17,Jimmy Eng写道:
>
> Consider updating to "comet.2015011.win64.exe" from 
> "comet_binaries_2015011.zip" as searches should run faster.  The parameters 
> file will be slightly changed though so keep that in  mind.  Good luck.
>
> On Sat, Apr 18, 2015 at 2:17 AM, c qin <[email protected] <javascript:>> 
> wrote:
>
>> In fact, there is no error message, it was just stop and no changed when 
>> I click the "refresh". 
>> Because I attempt to repeat a process used the TPPv4.7.1 with 
>> comet2014010, as you said, I downloaded "comet_binaries_2014010.zip"and 
>> replaced the original comet.exe (C:\Inetpub\tpp-bin\) with 
>> comet.2014010.win64.exe 
>> from the zip,and rename it to "comet.exe". 
>> Surprisedly, as I restarted TPP and performed comet, it run well and 
>> completely! I clicked the "refresh" and it showed "search end" and "command 
>> successful"!
>> Gratitude beyond words, you help me a lot!
>>
>>
>>
>> 在 2015年4月15日星期三 UTC+8下午10:24:45,Jimmy Eng写道:
>>>
>>> Unless you post an error or log message or more descriptive information, 
>>> no one here will have any idea why your job "seems to be dead again".  But 
>>> you can try the following to see if it addresses the issues.
>>>
>>> Try setting  "spectrum_batch_size = 3000" and run again and see if that 
>>> changes anything.
>>>
>>> Another option to try is to update your Comet binary to the latest 
>>> version 2015.01 rev. 1 as the latest TPP distributes version 2014.02 rev. 
>>> 2.  Grab the file "comet_binaries_2015011.zip" from 
>>> http://sourceforge.net/projects/comet-ms/files/.  I believe the binary 
>>> you need to replace is at c:\inetpub\tpp-bin\comet.exe.  With this 2015.01 
>>> version, use these settings:
>>>
>>>    fragment_bin_tol = 0.01
>>>    use_sparse_matrix = 1
>>>    spectrum_batch_size = 25000
>>>
>>> Good luck.
>>>
>>> On Wed, Apr 15, 2015 at 2:01 AM, c qin <[email protected]> wrote:
>>>
>>>> Thank you so much. 
>>>> It is high resolution data. it is the first time I use TPP and Comet, 
>>>> and I just do this under the guidance of the article, the parameter file 
>>>> is 
>>>> dowloaded from PRIDE database and the data set identifier is PXD001126.
>>>>
>>>> fragment_bin_tol = 0.1
>>>>
>>>> use_sparse_matrix = 0
>>>>
>>>> spectrum_batch_size = 5000
>>>>
>>>>
>>>> I perform TPP on win7(64bit) with  16G memory...
>>>>
>>>> 在 2015年4月15日星期三 UTC+8下午1:08:02,Jimmy Eng写道:
>>>>>
>>>>> Is this high-res MS/MS data?  If so, what are your settings for the 
>>>>> following Comet search parameters?
>>>>>
>>>>>    fragment_bin_tol
>>>>>    use_sparse_matrix
>>>>>    spectrum_batch_size
>>>>>
>>>>> And how much memory (RAM) does your computer have?
>>>>>
>>>>> - Jimmy
>>>>>    
>>>>>
>>>>> On Tue, Apr 14, 2015 at 7:47 PM, c qin <[email protected]> wrote:
>>>>>
>>>>>> Thanks for your attention
>>>>>> now I can kill and delete my jobs,but when I start an other new 
>>>>>> job,it seems to be dead again,and the output file size stop being 
>>>>>> increased.
>>>>>> I`m sure it is not finished because the output file does not reach 
>>>>>> expected size like my reference artical showed...
>>>>>> Do you encounter this problem?
>>>>>>
>>>>>>
>>>>>> 在 2015年4月13日星期一 UTC+8下午12:35:43,andyzcq写道:
>>>>>>>
>>>>>>> Hi, I came across this issue and solved it in the following way.
>>>>>>> Find the TPP username folder (normally should 
>>>>>>> be C:\inetpub\tpp-bin\users\guest), and delete the file the name of 
>>>>>>> which contains the date of task.
>>>>>>> Note: do not delete .jobs and .password files.
>>>>>>> Hope it helps.
>>>>>>>
>>>>>>> On Sun, Apr 12, 2015 at 3:57 PM, c qin <[email protected]> wrote:
>>>>>>>
>>>>>>>>
>>>>>>>> *Hello,*
>>>>>>>>
>>>>>>>> Can I ask a question about TPP(Trans Proteomic Pipeline)?
>>>>>>>>
>>>>>>>> I run a job on the web of TPP several days ago,and it is running in 
>>>>>>>> Database Search tab of Analysis Pipeline (Comet).However, until now,it 
>>>>>>>> is 
>>>>>>>> still in the running……
>>>>>>>> My data size is about 3GB,and I do not feel it is too big. When I 
>>>>>>>> click the “kill job” button,a messages show on orange window and tell 
>>>>>>>> that “Unable 
>>>>>>>> to kill command 3340! ()” 
>>>>>>>> I have no idea what should I do to finish(continue or kill) my 
>>>>>>>> job...
>>>>>>>>
>>>>>>>>
>>>>>>>> Thank you
>>>>>>>> c qin
>>>>>>>>
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