Hi,

I send you the mzxml file through wetransfer.
I tried with this command
* /opt/tpp/bin/PTMProphetParser STY,79.966,K,14.0156,M,15.995 MZTOL=0.2
/opt/tpp/data/327_05_01/327_05_01_020_150417_01_MTCS_0_D_interact.iproph.pep.xml
interact.ptm.pep.xml *

I got these warnings:

INFO: Writing file interact.ptm.pep.xml ...
INFO: Reading file
/opt/tpp/data/327_05_01/327_05_01_020_150417_01_MTCS_0_D_interact.iproph.pep.xml
...WARNING: Illegal peptide with unknown mod:
GTAGK[142]VIK[142]WARNING: Illegal peptide with unknown mod:
ILVAIM[147]K[142]WARNING: Illegal peptide with unknown mod:
VTNLHVK[142]WARNING: Illegal peptide with unknown mod:
ALK[142]EPPRWARNING: Illegal peptide with unknown mod:
ALK[142]EPPRWARNING: Illegal peptide with unknown mod:
ALK[142]EPPRWARNING: Illegal peptide with unknown mod:
K[142]EGVK[142]PRWARNING: Illegal peptide with unknown mod:
VLEPENK[142]WARNING: Illegal peptide with unknown mod:
VLNILEK[142]WARNING: Illegal peptide with unknown mod:
WLVVPDK[142]WARNING: Illegal peptide with unknown mod:
LLLTTPAK[142]WARNING: Illegal peptide with unknown mod:
K[142]PHPPKRWARNING: Illegal peptide with unknown mod:
KPHPPK[142]RWARNING: Illegal peptide with unknown mod:
DADFNGTK[142]WARNING: Illegal peptide with unknown mod:
LYSC[160]TPRWARNING: Illegal peptide with unknown mod:
KAVVVC[160]PKWARNING: Illegal peptide with unknown mod:
KTPM[147]C[160]EKWARNING: Illegal peptide with unknown mod:
LAALAEALK[142]WARNING: Illegal peptide with unknown mod:
VK[142]THLFRWARNING: Illegal peptide with unknown mod:
M[147]EFLKQKWARNING: Illegal peptide with unknown mod:
M[147]DK[142]TFERWARNING: Illegal peptide with unknown mod:
GAGM[147]SFSRKWARNING: Illegal peptide with unknown mod:
ALLVYC[160]VKWARNING: Illegal peptide with unknown mod:
ALLVYC[160]VKWARNING: Illegal peptide with unknown mod:
VFISC[160]K[142]VQWARNING: Illegal peptide with unknown mod:
VM[147]LYPSRIWARNING: Illegal peptide with unknown mod:
M[147]C[160]GDC[160]VEKWARNING: Illegal peptide with unknown mod:
LSLHLSPIK[142]WARNING: Illegal peptide with unknown mod:
M[147]SVNISTAGKWARNING: Illegal peptide with unknown mod:
NLMANRPAK[142]WARNING: Illegal peptide with unknown mod:
TAM[147]LLALQRWARNING: Illegal peptide with unknown mod:
LSSC[160]KPPKKWARNING: Illegal peptide with unknown mod:
K[142]QLYPFFKWARNING: Illegal peptide with unknown mod:
KQLYPFFK[142]WARNING: Illegal peptide with unknown mod:
TK[142]LNYNPPKWARNING: Illegal peptide with unknown mod:
TKLNYNPPK[142]WARNING: Illegal peptide with unknown mod:
IIK[142]ALDLPAKWARNING: Illegal peptide with unknown mod:
IIKALDLPAK[142]WARNING: Illegal peptide with unknown mod:
VK[142]PNVAVLSRWARNING: Illegal peptide with unknown mod:
ASSLPSSAPAVK[142]WARNING: Illegal peptide with unknown mod:
SLASSAQPGLGK[142]WARNING: Illegal peptide with unknown mod:
AEM[147]EELM[147]EKWARNING: Illegal peptide with unknown mod:
NDC[160]TTQSNVKWARNING: Illegal peptide with unknown mod:
LNK[142]TPIPQTK[142]WARNING: Illegal peptide with unknown mod:
SSLLGTGITSPK[142]WARNING: Illegal peptide with unknown mod:
LIDLC[160]QPTQK[142]WARNING: Illegal peptide with unknown mod:
RNQDRPSLLK[142]WARNING: Illegal peptide with unknown mod:
K[142]RPDEMLLPKWARNING: Illegal peptide with unknown mod:
KRPDEMLLPK[142]WARNING: Illegal peptide with unknown mod:
LAERPNIKM[147]RWARNING: Illegal peptide with unknown mod:
K[142]ITGEIMHALKWARNING: Illegal peptide with unknown mod:
KITGEIMHALK[142]WARNING: Illegal peptide with unknown mod:
NVQTPQC[160]K[142]LRWARNING: Illegal peptide with unknown mod:
VGAWGISGEPRK[142]WARNING: Illegal peptide with unknown mod:
DLLHPSPEEEK[142]WARNING: Illegal peptide with unknown mod:
GALGPGGPLGHPGEK[142]WARNING: Illegal peptide with unknown mod:
KAGTVM[147]FEYGMRWARNING: Illegal peptide with unknown mod:
KAGTVMFEYGM[147]RWARNING: Illegal peptide with unknown mod:
REENQNEVNM[147]KWARNING: Illegal peptide with unknown mod:
K[142]NSGC[160]TEVC[160]HTRWARNING: Illegal peptide with unknown mod:
QILLLLPVM[147]INM[147]LK[142]WARNING: Illegal peptide with unknown
mod: QILLLLPVM[147]INM[147]LK[142]WARNING: Illegal peptide with
unknown mod: LQGEGLSVAGIVC[160]HVGKWARNING: Illegal peptide with
unknown mod: SWAEAYK[142]DLENSDEFK[142]WARNING: Illegal peptide with
unknown mod: DLVSGGSNEGNGK[142]EDWAMK[142]WARNING: Illegal peptide
with unknown mod: AVATGKM[147]DENQFVAVTSTNAAKWARNING: Illegal peptide
with unknown mod: LPMASSMEHGK[142]DLPSVQLLMK[142]WARNING: Illegal
peptide with unknown mod: M[147]QILTPPLQSSVELVADPETRWARNING: Illegal
peptide with unknown mod: LTPTEIVLILPM[147]PEQRNSLTAK[142]WARNING:
Illegal peptide with unknown mod:
QKLC[160]YVSQGNASFTYGYEYLGC[160]TSRWARNING: Illegal peptide with
unknown mod: DNEIQMDMTLGPIEEAYAILNRFEVEVTK[142]WARNING: Illegal
peptide with unknown mod:
QM[147]GQEIGNELDEQNEIIDDLANLVENTDEK[142]WARNING: Illegal peptide with
unknown mod: HLVC[160]SFRLYPFTVHTVSPGNSHLALYQVFK[142]WARNING: Illegal
peptide with unknown mod:
EVK[142]VYLLTTSSAPYC[160]NAFQLLTVAPNHGISLK[142]WARNING: Illegal
peptide with unknown mod:
K[142]GNFQLQGTM[147]LSDGQGFTQDDVQAAEVTYGAMARWARNING: Illegal peptide
with unknown mod: K[142]GNFQLQGTMLSDGQGFTQDDVQAAEVTYGAM[147]ARWARNING:
Illegal peptide with unknown mod:
EESLHSILAGSDM[147]TVSQILLTQHGIPVM[147]NLSDGK[142]WARNING: Illegal
peptide with unknown mod:
DPPAQC[160]SAGPVGDDM[147]FHWQATIM[147]GPISNYMLDNWARNING: Illegal
peptide with unknown mod:
DPPAQC[160]SAGPVGDDM[147]FHWQATIMGPISNYM[147]LDNWARNING: Illegal
peptide with unknown mod:
DPPAQC[160]SAGPVGDDMFHWQATIM[147]GPISNYM[147]LDNWARNING: Illegal
peptide with unknown mod:
LYC[160]NGDGEWM[147]VPIGRC[160]TC[160]K[142]PGYEPENSVAC[160]KWARNING:
Illegal peptide with unknown mod:
LYC[160]NGDGEWM[147]VPIGRC[160]TC[160]KPGYEPENSVAC[160]K[142]WARNING:
Illegal peptide with unknown mod:
ASVDSGNEDIIEALISLFYGVM[147]TPMLNPLIYSLRWARNING: Illegal peptide with
unknown mod: ASVDSGNEDIIEALISLFYGVMTPM[147]LNPLIYSLRWARNING: Illegal
peptide with unknown mod:
M[147]GFNRPSKIQENALPLMLAEPPQNLIAQSQSGTGKWARNING: Illegal peptide with
unknown mod: MGFNRPSKIQENALPLM[147]LAEPPQNLIAQSQSGTGKWARNING: Illegal
peptide with unknown mod:
GFPSQC[160]YSAQTLPELC[160]SPQDELDFLM[147]EALIISKWARNING: Illegal
peptide with unknown mod:
LYTM[147]DGITVTVADLFFAGTETTSTTLRYGLLILMK[142]WARNING: Illegal peptide
with unknown mod:
LYTMDGITVTVADLFFAGTETTSTTLRYGLLILM[147]K[142]WARNING: Illegal peptide
with unknown mod:
FVHKSFVEVNEEGTEAAAASSC[160]FVVAEC[160]C[160]M[147]ESGPRWARNING:
Illegal peptide with unknown mod:
GPLLAQEM[147]PTVSVLEYALPQRPPQGPEDDRDLSER
Failed to open input file
'/opt/tpp/data/327_05_01/spectrast/327_05_01_020_150417_01.mzXML'.ERROR:
cannot read scan in data file
/opt/tpp/data/327_05_01/spectrast/327_05_01_020_150417_01.mzXML
exiting ...


There is an error at the end. I don't understand why it is looking for the
mzxml file in the spectrast folder... Thus I copied the mzxml file to the
spectrast folder and it seems to work.
Could you please check if the latest PTMprophet version gives the same
warnings?

Thanks for your help :-)

Delphine

2015-04-29 19:30 GMT+02:00 David Shteynberg <
[email protected]>:

> Hi,
>
> There may be several things going on here, which will require a new copy
> of PTMProphet for you to process the results.  I think that you are using
> an older version of PTMProphet which has seen recent improvements to cover
> more PTM user cases.  PTMProphet compares the internally calculated mass to
> the search engine reported mass, these may not agree and cause this problem
> for the K[142] peptides.  The other peptides are M containing with oxidized
> Methionine so you PTMProphet settings should include M,15.9949 to make
> those go away.  If you also send the mzXML file, I can try the latest code
> on you data.
>
>
> Thanks,
> -David
>
> On Wed, Apr 29, 2015 at 8:16 AM, Delphine <[email protected]> wrote:
>
>> Hello,
>>
>> I'm trying to use ptmprophet to identify methylation on lysines. First I
>> identify the peptides with comet, xtandem, spectraST and mascot. Here are
>> the lines where the modification is discribed in the xtandem and comet
>> parameter files I used:
>>  comet: variable_mod02 = 14.015650 K 0 3 -1 0
>>  xtandem: <note type="input" label="residue, potential modification
>> mass">15.994915@M,14.015650@K</note>
>>
>> I combine the 4 results with iprophet (file joined)  and tried to use
>> PTMprophet on this file.
>>
>> Here is the command line:
>> * cd /opt/tpp/data/327_05_01; /opt/tpp/bin/PTMProphetParser K,14.0156
>> MZTOL=0.2
>> /opt/tpp/data/327_05_01/327_05_01_020_150417_01_MTCS_0_D_interact.iproph.pep.xml
>> interact.ptm.pep.xml *
>>
>> And here is the error message:
>>
>> INFO: Writing file interact.ptm.pep.xml ...
>> INFO: Reading file 
>> /opt/tpp/data/327_05_01/327_05_01_020_150417_01_MTCS_0_D_interact.iproph.pep.xml
>>  ...WARNING: Illegal peptide with unknown mod: GTAGK[142]VIK[142]WARNING: 
>> Illegal peptide with unknown mod: ILVAIMK[142]WARNING: Illegal peptide with 
>> unknown mod: VTNLHVK[142]WARNING: Illegal peptide with unknown mod: 
>> ALK[142]EPPRWARNING: Illegal peptide with unknown mod: ALK[142]EPPRWARNING: 
>> Illegal peptide with unknown mod: ALK[142]EPPRWARNING: Illegal peptide with 
>> unknown mod: K[142]EGVK[142]PRWARNING: Illegal peptide with unknown mod: 
>> VLEPENK[142]WARNING: Illegal peptide with unknown mod: VLNILEK[142]WARNING: 
>> Illegal peptide with unknown mod: WLVVPDK[142]WARNING: Illegal peptide with 
>> unknown mod: LLLTTPAK[142]WARNING: Illegal peptide with unknown mod: 
>> K[142]PHPPKRWARNING: Illegal peptide with unknown mod: KPHPPK[142]RWARNING: 
>> Illegal peptide with unknown mod: DADFNGTK[142]WARNING: Illegal peptide with 
>> unknown mod: LYSC[160]TPRWARNING: Illegal peptide with unknown mod: 
>> KAVVVC[160]PKWARNING: Illegal peptide with unknown mod: KTPMC[160]EKWARNING: 
>> Illegal peptide with unknown mod: LAALAEALK[142]WARNING: Illegal peptide 
>> with unknown mod: VK[142]THLFRWARNING: Illegal peptide with unknown mod: 
>> MEFLKQKWARNING: Illegal peptide with unknown mod: MDK[142]TFERWARNING: 
>> Illegal peptide with unknown mod: GAGMSFSRKWARNING: Illegal peptide with 
>> unknown mod: ALLVYC[160]VKWARNING: Illegal peptide with unknown mod: 
>> ALLVYC[160]VKWARNING: Illegal peptide with unknown mod: 
>> VFISC[160]K[142]VQWARNING: Illegal peptide with unknown mod: 
>> VMLYPSRIWARNING: Illegal peptide with unknown mod: 
>> MC[160]GDC[160]VEKWARNING: Illegal peptide with unknown mod: 
>> LSLHLSPIK[142]WARNING: Illegal peptide with unknown mod: MSVNISTAGKWARNING: 
>> Illegal peptide with unknown mod: NLMANRPAK[142]WARNING: Illegal peptide 
>> with unknown mod: TAMLLALQRWARNING: Illegal peptide with unknown mod: 
>> LSSC[160]KPPKKWARNING: Illegal peptide with unknown mod: 
>> K[142]QLYPFFKWARNING: Illegal peptide with unknown mod: 
>> KQLYPFFK[142]WARNING: Illegal peptide with unknown mod: 
>> TK[142]LNYNPPKWARNING: Illegal peptide with unknown mod: 
>> TKLNYNPPK[142]WARNING: Illegal peptide with unknown mod: 
>> IIK[142]ALDLPAKWARNING: Illegal peptide with unknown mod: 
>> IIKALDLPAK[142]WARNING: Illegal peptide with unknown mod: 
>> VK[142]PNVAVLSRWARNING: Illegal peptide with unknown mod: 
>> ASSLPSSAPAVK[142]WARNING: Illegal peptide with unknown mod: 
>> SLASSAQPGLGK[142]WARNING: Illegal peptide with unknown mod: 
>> AEMEELMEKWARNING: Illegal peptide with unknown mod: NDC[160]TTQSNVKWARNING: 
>> Illegal peptide with unknown mod: LNK[142]TPIPQTK[142]WARNING: Illegal 
>> peptide with unknown mod: SSLLGTGITSPK[142]WARNING: Illegal peptide with 
>> unknown mod: LIDLC[160]QPTQK[142]WARNING: Illegal peptide with unknown mod: 
>> RNQDRPSLLK[142]WARNING: Illegal peptide with unknown mod: 
>> K[142]RPDEMLLPKWARNING: Illegal peptide with unknown mod: 
>> KRPDEMLLPK[142]WARNING: Illegal peptide with unknown mod: LAERPNIKMRWARNING: 
>> Illegal peptide with unknown mod: K[142]ITGEIMHALKWARNING: Illegal peptide 
>> with unknown mod: KITGEIMHALK[142]WARNING: Illegal peptide with unknown mod: 
>> NVQTPQC[160]K[142]LRWARNING: Illegal peptide with unknown mod: 
>> VGAWGISGEPRK[142]WARNING: Illegal peptide with unknown mod: 
>> DLLHPSPEEEK[142]WARNING: Illegal peptide with unknown mod: 
>> GALGPGGPLGHPGEK[142]WARNING: Illegal peptide with unknown mod: 
>> KAGTVMFEYGMRWARNING: Illegal peptide with unknown mod: REENQNEVNMKWARNING: 
>> Illegal peptide with unknown mod: K[142]NSGC[160]TEVC[160]HTRWARNING: 
>> Illegal peptide with unknown mod: QILLLLPVMINMLK[142]WARNING: Illegal 
>> peptide with unknown mod: QILLLLPVMINMLK[142]WARNING: Illegal peptide with 
>> unknown mod: LQGEGLSVAGIVC[160]HVGKWARNING: Illegal peptide with unknown 
>> mod: SWAEAYK[142]DLENSDEFK[142]WARNING: Illegal peptide with unknown mod: 
>> DLVSGGSNEGNGK[142]EDWAMK[142]WARNING: Illegal peptide with unknown mod: 
>> AVATGKMDENQFVAVTSTNAAKWARNING: Illegal peptide with unknown mod: 
>> LPMASSMEHGK[142]DLPSVQLLMK[142]WARNING: Illegal peptide with unknown mod: 
>> MQILTPPLQSSVELVADPETRWARNING: Illegal peptide with unknown mod: 
>> LTPTEIVLILPMPEQRNSLTAK[142]WARNING: Illegal peptide with unknown mod: 
>> QKLC[160]YVSQGNASFTYGYEYLGC[160]TSRWARNING: Illegal peptide with unknown 
>> mod: DNEIQMDMTLGPIEEAYAILNRFEVEVTK[142]WARNING: Illegal peptide with unknown 
>> mod: QMGQEIGNELDEQNEIIDDLANLVENTDEK[142]WARNING: Illegal peptide with 
>> unknown mod: HLVC[160]SFRLYPFTVHTVSPGNSHLALYQVFK[142]WARNING: Illegal 
>> peptide with unknown mod: 
>> EVK[142]VYLLTTSSAPYC[160]NAFQLLTVAPNHGISLK[142]WARNING: Illegal peptide with 
>> unknown mod: K[142]GNFQLQGTMLSDGQGFTQDDVQAAEVTYGAMARWARNING: Illegal peptide 
>> with unknown mod: EESLHSILAGSDMTVSQILLTQHGIPVMNLSDGK[142]WARNING: Illegal 
>> peptide with unknown mod: DPPAQC[160]SAGPVGDDMFHWQATIMGPISNYMLDNWARNING: 
>> Illegal peptide with unknown mod: 
>> LYC[160]NGDGEWMVPIGRC[160]TC[160]K[142]PGYEPENSVAC[160]KWARNING: Illegal 
>> peptide with unknown mod: 
>> LYC[160]NGDGEWMVPIGRC[160]TC[160]KPGYEPENSVAC[160]K[142]WARNING: Illegal 
>> peptide with unknown mod: ASVDSGNEDIIEALISLFYGVMTPMLNPLIYSLRWARNING: Illegal 
>> peptide with unknown mod: MGFNRPSKIQENALPLMLAEPPQNLIAQSQSGTGKWARNING: 
>> Illegal peptide with unknown mod: 
>> GFPSQC[160]YSAQTLPELC[160]SPQDELDFLMEALIISKWARNING: Illegal peptide with 
>> unknown mod: LYTMDGITVTVADLFFAGTETTSTTLRYGLLILMK[142]WARNING: Illegal 
>> peptide with unknown mod: 
>> FVHKSFVEVNEEGTEAAAASSC[160]FVVAEC[160]C[160]MESGPRWARNING: Illegal peptide 
>> with unknown mod: GPLLAQEMPTVSVLEYALPQRPPQGPEDDRDLSERWARNING: Illegal 
>> peptide with unknown mod: GGEC[160]HGMDAEQEMRWARNING: Illegal peptide with 
>> unknown mod: WAAVMVPSGEEQRWARNING: Illegal peptide with unknown mod: 
>> MTSMMC[160]TVMLK[142]TWARNING: Illegal peptide with unknown mod: 
>> MSFSEMNRWARNING: Illegal peptide with unknown mod: MSFAGTVAWMAPEVIRWARNING: 
>> Illegal peptide with unknown mod: GSQDFSFREIMGSRWARNING: Illegal peptide 
>> with unknown mod: RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with 
>> unknown mod: RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown 
>> mod: EMSEEMDK[142]WARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> MVLLAGTGPEGGGARWARNING: Illegal peptide with unknown mod: 
>> QDFNMMEQRK[142]WARNING: Illegal peptide with unknown mod: 
>> MEAMGEWNPNVKWARNING: Illegal peptide with unknown mod: 
>> AVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> TK[142]EMDVYGITDKWARNING: Illegal peptide with unknown mod: 
>> TKEMDVYGITDK[142]WARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> VMAMAIDYRWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> AVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> AVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> IKFEMEQNLRWARNING: Illegal peptide with unknown mod: MNGLRNKWARNING: Illegal 
>> peptide with unknown mod: RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide 
>> with unknown mod: AVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with 
>> unknown mod: AVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown 
>> mod: AVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> VMLYPSRWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> VMLYPSRWARNING: Illegal peptide with unknown mod: KLIMEAMKWARNING: Illegal 
>> peptide with unknown mod: MC[160]GDC[160]VEKWARNING: Illegal peptide with 
>> unknown mod: SESMDYSRWARNING: Illegal peptide with unknown mod: 
>> GMPGGRNLYKWARNING: Illegal peptide with unknown mod: 
>> AVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> MNAFYDAQVEFVKWARNING: Illegal peptide with unknown mod: IITVMSMGMKWARNING: 
>> Illegal peptide with unknown mod: LNIFDMMAVTKWARNING: Illegal peptide with 
>> unknown mod: RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown 
>> mod: DVDNAYMIKWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> MNWENESSPKWARNING: Illegal peptide with unknown mod: LNIFDMMAVTKWARNING: 
>> Illegal peptide with unknown mod: YYADGEDAYAMKWARNING: Illegal peptide with 
>> unknown mod: APAMFNIRWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> APAMFNIRWARNING: Illegal peptide with unknown mod: GFMVQTGDPTGTGRWARNING: 
>> Illegal peptide with unknown mod: RAVGIWHC[160]GSC[160]MKWARNING: Illegal 
>> peptide with unknown mod: MTLDDFRWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> QVLDNLTMEKWARNING: Illegal peptide with unknown mod: QVLDNLTMEKWARNING: 
>> Illegal peptide with unknown mod: RAVGIWHC[160]GSC[160]MKWARNING: Illegal 
>> peptide with unknown mod: RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide 
>> with unknown mod: QVLDNLTMEKWARNING: Illegal peptide with unknown mod: 
>> NTPGFMYK[142]WARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> EGMLMMMSPSPRWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> RAVGIWHC[160]GSC[160]MKWARNING: Illegal peptide with unknown mod: 
>> K[142]ALMPPVKWARNING: Illegal peptide with unknown mod: 
>> KALMPPVK[142]WARNING: Illegal peptide with unknown mod: 
>> K[142]PVMPK[142]KWARNING: Illegal peptide with unknown mod: 
>> K[142]PVMPKK[142]WARNING: Illegal peptide with unknown mod: 
>> KPVMPK[142]K[142]WARNING: Illegal peptide with unknown mod: 
>> K[142]PVMPK[142]KWARNING: Illegal peptide with unknown mod: 
>> K[142]PVMPKK[142]WARNING: Illegal peptide with unknown mod: 
>> KPVMPK[142]K[142]WARNING: Illegal peptide with unknown mod: 
>> VTMQK[142]SDDVLHDLGQKWARNING: Illegal peptide with unknown mod: 
>> VTMQKSDDVLHDLGQK[142]WARNING: Illegal peptide with unknown mod: 
>> VMLYPSRIWARNING: Illegal peptide with unknown mod: VMLYPSRIWARNING: Illegal 
>> peptide with unknown mod: VMLYPSRIWARNING: Illegal peptide with unknown mod: 
>> VMLYPSRIWARNING: Illegal peptide with unknown mod: MAGGLFAVSKKWARNING: 
>> Illegal peptide with unknown mod: IGQQLGMTFISVGHRWARNING: Illegal peptide 
>> with unknown mod: VMLYPSRIWARNING: Illegal peptide with unknown mod: 
>> VMLYPSRIWARNING: Illegal peptide with unknown mod: K[142]PVMPK[142]KWARNING: 
>> Illegal peptide with unknown mod: K[142]PVMPKK[142]WARNING: Illegal peptide 
>> with unknown mod: KPVMPK[142]K[142]WARNING: Illegal peptide with unknown 
>> mod: VMLYPSRIWARNING: Illegal peptide with unknown mod: 
>> LEVVAIFGSVQMAMSRIINLHHHRWARNING: Illegal peptide with unknown mod: 
>> GLIFYDTKVTVMNRWARNING: Illegal peptide with unknown mod: 
>> LK[142]LSHLVQYEELPDYIMVMVANK[142]WARNING: Illegal peptide with unknown mod: 
>> AVLVDLEPGTMDSVRWARNING: Illegal peptide with unknown mod: 
>> AVLVDLEPGTMDSVRWARNING: Illegal peptide with unknown mod: TMADQQARR
>>      WARNING: Unrecognized mod on peptide: TVVGQITVDM[147]WARNING: Cannot 
>> initialize for sequence: TVVGQITVDM[147], unknown mods may exist in spectrum 
>> 327_05_01_020_150417_01.05584.05584.2
>> Segmentation fault
>>
>>
>> I am not very familliar with the search of PTM....
>> Thanks in advance for your help!
>>
>> Delphine
>>
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