As the error message suggests, SpectraST doesn’t know about the
modification you specified:
Peptide ID has unknown modification: "n[272]CPRAAR".
n[272] is not known. I thought TMT6plex is n[230]. You seem to be adding a
very different mass to the n terminus than the TMT6 that I know, or I’m
confused. Maybe your n-terminal mass mod is wrong? Or maybe you’re not
using TMT6?
The other numbers you show are very puzzling:
E,Glu->pyro-Glu: 215
K,TMT6plex: 197
Q,Gln->pyro-Glu: 101
n,TMT6plex: 2879
K,TMT6plex should be about half of n,TMT6plex and it’s not. And a scenario
where E,Glu->pyro-Glu is greater than K,TMT6plex is hard to fathom. These
relative counts don’t make sense.
I suggest checking your mass mods for the original search very carefully
and redoing the search.
Regards,
Eric
*From:* [email protected] [mailto:
[email protected]] *On Behalf Of *Ali
*Sent:* Wednesday, August 31, 2016 5:52 PM
*To:* spctools-discuss <[email protected]>
*Subject:* [spctools-discuss] Re: Problem when Creating a library in
Spectrast
Here there is more detail:
Total number of spectra in library: 2879
Total number of distinct peptide ions in library: 2349
Total number of distinct stripped peptides in library: 2298
CHARGE +1: 0 ; +2: 2098 ; +3: 781 ; +4: 0 ; +5: 0 ; >+5: 0 ;
Unk: 0
TERMINI Tryptic: 2404 ; Semi-tryptic: 462 ; Non-tryptic: 13
PROBABILITY >0.9999: 61 ; 0.999-0.9999: 1236 ; 0.99-0.999: 936 ;
0.9-0.99: 421 ; <0.9: 225
NREPS 20+: 0 ; 10-19: 0 ; 4-9: 0 ; 2-3: 0 ; 1: 2879
MODIFICATIONS E,Glu->pyro-Glu: 215
K,TMT6plex: 197
Q,Gln->pyro-Glu: 101
n,TMT6plex: 2879
On Wednesday, August 31, 2016 at 8:48:09 PM UTC-4, Ali wrote:
Hi
I want to create a library from a pep.xml file which is the result of X!
Tandem search (I converted the X!Tandem to pep.xml using Tandem2XML) using
spectrast with the following simple command:
spectrast -cN1607011A_NC20A_10ul_180min.splib -cP0.9
1607011A_NC20A_10ul_180min.tandem.pep.xml
I receive the following error:
SpectraST finished at Wed Aug 31 15:08:36 2016 with 195 error(s):
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]CPRAAR". Skipped
spectrum 1607011A_NC20A_10ul_180min.02812.02812.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MAAAAAQGGGGGEPR". Skipped spectrum
1607011A_NC20A_10ul_180min.02849.02849.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MSGGGGGGGSAPSR".
Skipped spectrum 1607011A_NC20A_10ul_180min.03003.03003.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGGGQR". Skipped
spectrum 1607011A_NC20A_10ul_180min.03085.03085.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGLLGR". Skipped
spectrum 1607011A_NC20A_10ul_180min.03108.03108.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGGGQR". Skipped
spectrum 1607011A_NC20A_10ul_180min.03354.03354.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MAAAAGGGSCPGPGSAR". Skipped spectrum
1607011A_NC20A_10ul_180min.03413.03413.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MATANGAVENGQPDR". Skipped spectrum
1607011A_NC20A_10ul_180min.03452.03452.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGLVAR". Skipped
spectrum 1607011A_NC20A_10ul_180min.03464.03464.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]LAGGVR". Skipped
spectrum 1607011A_NC20A_10ul_180min.03465.03465.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MSVQVAAPGSAGLGPER". Skipped spectrum
1607011A_NC20A_10ul_180min.03771.03771.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]DAGSER". Skipped
spectrum 1607011A_NC20A_10ul_180min.03826.03826.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]LAGGVR". Skipped
spectrum 1607011A_NC20A_10ul_180min.03846.03846.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]IIGPRGPGSQR".
Skipped spectrum 1607011A_NC20A_10ul_180min.03857.03857.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]RRPRQGGGGAGGSAAAR". Skipped spectrum
1607011A_NC20A_10ul_180min.03870.03870.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]APGVAR". Skipped
spectrum 1607011A_NC20A_10ul_180min.03873.03873.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]PPCSGGDGSTPPGPSLR". Skipped spectrum
1607011A_NC20A_10ul_180min.03903.03903.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MNNAGHGPTRR".
Skipped spectrum 1607011A_NC20A_10ul_180min.03906.03906.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]VSSGCR". Skipped
spectrum 1607011A_NC20A_10ul_180min.04014.04014.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]EDAAAPGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.04033.04033.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]ATGGDDPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.04047.04047.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]DGGAASQDESSAAAAAAADSR". Skipped spectrum
1607011A_NC20A_10ul_180min.04240.04240.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]ACAGSTR".
Skipped spectrum 1607011A_NC20A_10ul_180min.04383.04383.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MGGGGSALR".
Skipped spectrum 1607011A_NC20A_10ul_180min.04389.04389.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]LASGPAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.04425.04425.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]DASGRAAAEGWR".
Skipped spectrum 1607011A_NC20A_10ul_180min.04557.04557.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MHPAGLAAAAAGTPR". Skipped spectrum
1607011A_NC20A_10ul_180min.04586.04586.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]PEAGATLR".
Skipped spectrum 1607011A_NC20A_10ul_180min.04738.04738.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MGTAAAAAAAAAAAAAGEGAR". Skipped spectrum
1607011A_NC20A_10ul_180min.05039.05039.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAAGPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.05094.05094.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]PGGPSPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.05100.05100.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]ERGGAGGGPGGAGGGSGQR". Skipped spectrum
1607011A_NC20A_10ul_180min.05205.05205.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]ERGGAGGGPGGAGGGSGQR". Skipped spectrum
1607011A_NC20A_10ul_180min.05214.05214.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]ERGGAGGGPGGAGGGSGQR". Skipped spectrum
1607011A_NC20A_10ul_180min.05221.05221.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]ERGGAGGGPGGAGGGSGQR". Skipped spectrum
1607011A_NC20A_10ul_180min.05235.05235.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]GTAAAAAAAAAAAAAGEGAR". Skipped spectrum
1607011A_NC20A_10ul_180min.05378.05378.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GAAGSDGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.05586.05586.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MSVQVAAPGSAGLGPER". Skipped spectrum
1607011A_NC20A_10ul_180min.05662.05662.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAPPAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.05706.05706.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAPVAAGSGAGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.05716.05716.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MAAAAGGGSCPGPGSAR". Skipped spectrum
1607011A_NC20A_10ul_180min.05833.05833.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MGSVGSQR".
Skipped spectrum 1607011A_NC20A_10ul_180min.06095.06095.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAAAQSR".
Skipped spectrum 1607011A_NC20A_10ul_180min.06135.06135.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGSGAGVR".
Skipped spectrum 1607011A_NC20A_10ul_180min.06378.06378.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MDSGAGGRR".
Skipped spectrum 1607011A_NC20A_10ul_180min.06449.06449.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MGRAAATAGGGGGARR". Skipped spectrum
1607011A_NC20A_10ul_180min.06460.06460.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MSSGGGSR".
Skipped spectrum 1607011A_NC20A_10ul_180min.06471.06471.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]DPAPAR". Skipped
spectrum 1607011A_NC20A_10ul_180min.06521.06521.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MGPGPSR".
Skipped spectrum 1607011A_NC20A_10ul_180min.06526.06526.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SSCSGLSR".
Skipped spectrum 1607011A_NC20A_10ul_180min.06577.06577.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]PGGAGAAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.06584.06584.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GFGPGLTAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.06635.06635.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MEPGSAEMPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.06742.06742.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MRSGAAPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.06824.06824.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SGAAGAAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.06871.06871.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]AAAAAGLGGGGAGPGPEAGDFLAR". Skipped spectrum
1607011A_NC20A_10ul_180min.06879.06879.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GAAAESGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.06903.06903.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAVAAALAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.06951.06951.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAIPALD".
Skipped spectrum 1607011A_NC20A_10ul_180min.06954.06954.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGTTSTRR".
Skipped spectrum 1607011A_NC20A_10ul_180min.07004.07004.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAGAAPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.07207.07207.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MASDAVQSEPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.07208.07208.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MAAPAGGGGSAVSVLAPNGR". Skipped spectrum
1607011A_NC20A_10ul_180min.07211.07211.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]FGGPGPGVLGAQGMAGPLR". Skipped spectrum
1607011A_NC20A_10ul_180min.07272.07272.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]RPLAGGSR".
Skipped spectrum 1607011A_NC20A_10ul_180min.07288.07288.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SGAAGAAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.07350.07350.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MGGCAGSR".
Skipped spectrum 1607011A_NC20A_10ul_180min.07380.07380.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]PSGEER". Skipped
spectrum 1607011A_NC20A_10ul_180min.07404.07404.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MSAPVGPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.07444.07444.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]ATSGDCPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.07583.07583.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MGPGPSR".
Skipped spectrum 1607011A_NC20A_10ul_180min.07610.07610.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAEGGFD".
Skipped spectrum 1607011A_NC20A_10ul_180min.07708.07708.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AVCGGAAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.07724.07724.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]VSVPGPAAAAAPAAGR". Skipped spectrum
1607011A_NC20A_10ul_180min.07838.07838.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SPAAAAAGAGER".
Skipped spectrum 1607011A_NC20A_10ul_180min.07988.07988.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MSPGASR".
Skipped spectrum 1607011A_NC20A_10ul_180min.08063.08063.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAGAGAPGNPED".
Skipped spectrum 1607011A_NC20A_10ul_180min.08072.08072.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAGAGAPGNPED".
Skipped spectrum 1607011A_NC20A_10ul_180min.08094.08094.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]DGVAGPQLGAAAEAAEAAEAR". Skipped spectrum
1607011A_NC20A_10ul_180min.08098.08098.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGAWGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.08180.08180.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MPGEAAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.08206.08206.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]AAPYPGSGGGSEVK[357]". Skipped spectrum
1607011A_NC20A_10ul_180min.08321.08321.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MAVAVGVRAAPVLGLAR". Skipped spectrum
1607011A_NC20A_10ul_180min.08406.08406.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGSGAGVR".
Skipped spectrum 1607011A_NC20A_10ul_180min.08430.08430.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGSGAGVR".
Skipped spectrum 1607011A_NC20A_10ul_180min.08433.08433.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SPEAQPQR".
Skipped spectrum 1607011A_NC20A_10ul_180min.08477.08477.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SGFSTEER".
Skipped spectrum 1607011A_NC20A_10ul_180min.08492.08492.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GNAPSQD".
Skipped spectrum 1607011A_NC20A_10ul_180min.08511.08511.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGGVCVGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.08531.08531.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGSGAGVR".
Skipped spectrum 1607011A_NC20A_10ul_180min.08548.08548.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAAVAGMLR".
Skipped spectrum 1607011A_NC20A_10ul_180min.08552.08552.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]EAAVGVPDGGDQGGAGPR". Skipped spectrum
1607011A_NC20A_10ul_180min.08642.08642.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAAGGAASR".
Skipped spectrum 1607011A_NC20A_10ul_180min.08768.08768.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]ALNVAPVR".
Skipped spectrum 1607011A_NC20A_10ul_180min.08854.08854.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MAAAEEGCSVGAEADR". Skipped spectrum
1607011A_NC20A_10ul_180min.08971.08971.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]PGILVGGAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.08998.08998.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAAGAAVAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09035.09035.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MHGGGPPSGDSACPLR". Skipped spectrum
1607011A_NC20A_10ul_180min.09094.09094.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]AAQGVGPGPGSAAPPGLEAAR". Skipped spectrum
1607011A_NC20A_10ul_180min.09099.09099.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAAAGGAASR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09119.09119.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SGAAGAAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09199.09199.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAAEER". Skipped
spectrum 1607011A_NC20A_10ul_180min.09255.09255.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]PGILVGGAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09269.09269.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGAWGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09348.09348.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]TGGAHSR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09380.09380.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]PNCSCAAGVSCTCASSCK[357]". Skipped spectrum
1607011A_NC20A_10ul_180min.09414.09414.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GPVPSR". Skipped
spectrum 1607011A_NC20A_10ul_180min.09426.09426.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MPGGPSPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09537.09537.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGAWGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09639.09639.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]AGPGSTGGQIGAAALAGGAR". Skipped spectrum
1607011A_NC20A_10ul_180min.09659.09659.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGSGAGVR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09694.09694.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGGPSGGGPGGSGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09722.09722.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGGPSGGGPGGSGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09723.09723.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAGGSAPEPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09727.09727.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MGGEQEEER".
Skipped spectrum 1607011A_NC20A_10ul_180min.09733.09733.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAGGSAPEPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09746.09746.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAGGSAPEPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09782.09782.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGSGAGVR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09801.09801.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAGGSAPEPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09855.09855.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GVSALLAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09869.09869.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGGPSGGGPGGSGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.09906.09906.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SGAGGRR".
Skipped spectrum 1607011A_NC20A_10ul_180min.10082.10082.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]AAAAAAAAAAGAAGGR". Skipped spectrum
1607011A_NC20A_10ul_180min.10143.10143.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]AAAAAAAAAAGAAGGR". Skipped spectrum
1607011A_NC20A_10ul_180min.10163.10163.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]AAAAAAAAAAGAAGGR". Skipped spectrum
1607011A_NC20A_10ul_180min.10324.10324.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]AAAAAAAAAAGAAGGR". Skipped spectrum
1607011A_NC20A_10ul_180min.10369.10369.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MPGPPALR".
Skipped spectrum 1607011A_NC20A_10ul_180min.10601.10601.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGGPSGGGPGGSGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.10854.10854.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGGPSGGGPGGSGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.10855.10855.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MASEASVR".
Skipped spectrum 1607011A_NC20A_10ul_180min.10910.10910.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGGPSGGGPGGSGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.10933.10933.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAVGSGGYAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.10976.10976.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MSSEDR". Skipped
spectrum 1607011A_NC20A_10ul_180min.10991.10991.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SGAAGAAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.11115.11115.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MASATED".
Skipped spectrum 1607011A_NC20A_10ul_180min.11259.11259.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]ASVAAAARR".
Skipped spectrum 1607011A_NC20A_10ul_180min.11363.11363.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AELGAGGDGHR".
Skipped spectrum 1607011A_NC20A_10ul_180min.11367.11367.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AELGAGGDGHR".
Skipped spectrum 1607011A_NC20A_10ul_180min.11369.11369.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MEEGAPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.11427.11427.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGSDPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.11445.11445.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]FLLGLR". Skipped
spectrum 1607011A_NC20A_10ul_180min.11584.11584.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGGGGSSK[357]".
Skipped spectrum 1607011A_NC20A_10ul_180min.11673.11673.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]LPPPPR". Skipped
spectrum 1607011A_NC20A_10ul_180min.11826.11826.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGSDPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.11957.11957.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]ELGAGGDGHR".
Skipped spectrum 1607011A_NC20A_10ul_180min.12159.12159.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GAAGAGAPGNPED".
Skipped spectrum 1607011A_NC20A_10ul_180min.12280.12280.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MELGGPGAPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.12308.12308.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGAWGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.12420.12420.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MELGGPGAPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.12496.12496.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGSDPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.12559.12559.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MASASGAMAK[357]". Skipped spectrum
1607011A_NC20A_10ul_180min.12580.12580.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GRAGGGGPGR".
Skipped spectrum 1607011A_NC20A_10ul_180min.12788.12788.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MSELADSGPSGASAPSQLR". Skipped spectrum
1607011A_NC20A_10ul_180min.12809.12809.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GSGGGAGER".
Skipped spectrum 1607011A_NC20A_10ul_180min.13091.13091.2 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MATSGANGPGSATASASNPR". Skipped spectrum
1607011A_NC20A_10ul_180min.13144.13144.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]ANGDSPPWSPALAAEGRGSSCEVR". Skipped spectrum
1607011A_NC20A_10ul_180min.13183.13183.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAGAGSEAR".
Skipped spectrum 1607011A_NC20A_10ul_180min.13366.13366.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]RSPGGILLQALPR".
Skipped spectrum 1607011A_NC20A_10ul_180min.13403.13403.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MGVALR". Skipped
spectrum 1607011A_NC20A_10ul_180min.13432.13432.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MATSFR". Skipped
spectrum 1607011A_NC20A_10ul_180min.13484.13484.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MTEPGASPED".
Skipped spectrum 1607011A_NC20A_10ul_180min.13555.13555.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MTEPGASPED".
Skipped spectrum 1607011A_NC20A_10ul_180min.13607.13607.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGAGGGPGLR".
Skipped spectrum 1607011A_NC20A_10ul_180min.13613.13613.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MTEPGASPED".
Skipped spectrum 1607011A_NC20A_10ul_180min.13725.13725.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]AALHTTPDSPAAQLER". Skipped spectrum
1607011A_NC20A_10ul_180min.13774.13774.3 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAAGPSTR".
Skipped spectrum 1607011A_NC20A_10ul_180min.13888.13888.2 .
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]RSGGGCSGSLSLR".
Skipped spectrum 1607011A_NC20A_10ul_180min.14320.14320.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]ATVVDAVNAAPLSGSK[357]". Skipped spectrum
1607011A_NC20A_10ul_180min.14447.14447.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MEAAFGQVAGSACPRR". Skipped spectrum
1607011A_NC20A_10ul_180min.14956.14956.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MAASAGAGAVIAAPDSR". Skipped spectrum
1607011A_NC20A_10ul_180min.15253.15253.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MGGAGALGGAGGVQGAAGR". Skipped spectrum
1607011A_NC20A_10ul_180min.15317.15317.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]TTFLQTSSSTFGGGSTR". Skipped spectrum
1607011A_NC20A_10ul_180min.15616.15616.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MLPWTALGLALSLRLALAR". Skipped spectrum
1607011A_NC20A_10ul_180min.16176.16176.3 .
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MASGAGGVGGGGGGK[357]"
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MVK[357]EGAAVIDVGINYV
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MRQGLLVLALVLVLVLVLAAG
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAIYGGVEGGGTR".
Skipp
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]LGSDDFFYVGGSPSTADLPGS
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]EGGPAVCCQDPRAELVER".
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]AAAAMAAAAGGGAGAARSLSR
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]LNGGGSGAGGSRGGGQER".
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]RRALLLLLLSFLAPWATIALR
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]APGALAQTAAPGPGR". Ski
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MAGDLSAGFFMEELNTYR".
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GAAARLSAPR".
Skipped
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]SSYDEASLAPEETTDSFWEVG
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]APRALPGSAVLAAAVFVGGAV
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MSHYGSYYGGLGYSCGGFGGL
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MDGASSGGGGSSEGGGGSSGS
PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGAAAGGR".
Skipped sp
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]SCARLLLLWGCTVVAAGLSGV
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]ASTSRDVIAGRGIHSK[357]
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]TESASCSTARQTK[357]".
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]MARAGWTGLLPLYVCLLLTCG
PEPXML IMPORT: Peptide ID has unknown modification:
"n[272]AAAAPAAAAASSEAPAASATA
Does any body knows what might be the problem? I am relatively new in TPP
and proteomics so I would appreciate if you could explain a little in
detail.
Thank you very much
--
You received this message because you are subscribed to the Google Groups
"spctools-discuss" group.
To unsubscribe from this group and stop receiving emails from it, send an
email to [email protected].
To post to this group, send email to [email protected].
Visit this group at https://groups.google.com/group/spctools-discuss.
For more options, visit https://groups.google.com/d/optout.
--
You received this message because you are subscribed to the Google Groups
"spctools-discuss" group.
To unsubscribe from this group and stop receiving emails from it, send an email
to [email protected].
To post to this group, send email to [email protected].
Visit this group at https://groups.google.com/group/spctools-discuss.
For more options, visit https://groups.google.com/d/optout.
