I went through these steps again and I am still getting the same error and everything in spectrast.
On Wednesday, August 31, 2016 at 11:04:39 PM UTC-4, Ali wrote: > > Thank you so much Eric for helping me. Let me tell you what I have done > before this: > > > - I have done the search with *TMT 10 plex of K* and *TMT 10plex of N > term* fixed residue modification in *X!Tandem*. The Enzyme was > Trypsin. I set the mass modification in X!Tandem as: > - <note label="residue, modification mass" > type="input">229.1629@K,229.1629@[</note> > > > - Then I converted the results of X! Tandem to pep.xml (Using > Tandem2XML) > > > - Next I got the probabilities with the following setting: > - PeptideProphetParser 1607011A_NC20A_10ul_180min.tandem.pep.xml > ACCMASS PPM NOICAT MINPROB=0.05 > > > and then I wanted to convert the result to splib that I encountered this > error. The modifications that spectrast gives me did not make sense to me > too. Do you have any idea what might have happened here? > > I am going to redo the search to see if anything changes. > > Thanks again! > > > > On Wednesday, August 31, 2016 at 10:21:17 PM UTC-4, Eric Deutsch wrote: >> >> Ah, one other idea. n[272] is probably n[230] plus n[42]. I suppose >> that’s TMT6plex plus Acetylation. Is that possible? If so, then maybe we >> need to teach SpectraST about it. But the relative ratios of the numbers I >> quoted below are still quite odd and raises a red flag. Is there a reason >> to expect so few peptides ending in lysine. Maybe it’s all fine. >> >> >> >> >> >> *From:* Eric Deutsch [mailto:[email protected]] >> *Sent:* Wednesday, August 31, 2016 7:15 PM >> *To:* [email protected] >> *Cc:* Eric Deutsch <[email protected]> >> *Subject:* RE: [spctools-discuss] Re: Problem when Creating a library in >> Spectrast >> >> >> >> As the error message suggests, SpectraST doesn’t know about the >> modification you specified: >> >> >> >> Peptide ID has unknown modification: "n[272]CPRAAR". >> >> >> >> n[272] is not known. I thought TMT6plex is n[230]. You seem to be adding >> a very different mass to the n terminus than the TMT6 that I know, or I’m >> confused. Maybe your n-terminal mass mod is wrong? Or maybe you’re not >> using TMT6? >> >> >> >> The other numbers you show are very puzzling: >> >> >> >> E,Glu->pyro-Glu: 215 >> >> K,TMT6plex: 197 >> >> Q,Gln->pyro-Glu: 101 >> >> n,TMT6plex: 2879 >> >> >> >> K,TMT6plex should be about half of n,TMT6plex and it’s not. And a >> scenario where E,Glu->pyro-Glu is greater than K,TMT6plex is hard to >> fathom. These relative counts don’t make sense. >> >> >> >> I suggest checking your mass mods for the original search very carefully >> and redoing the search. >> >> >> >> Regards, >> >> Eric >> >> >> >> >> >> >> >> *From:* [email protected] [mailto:[email protected]] >> *On Behalf Of *Ali >> *Sent:* Wednesday, August 31, 2016 5:52 PM >> *To:* spctools-discuss <[email protected]> >> *Subject:* [spctools-discuss] Re: Problem when Creating a library in >> Spectrast >> >> >> >> Here there is more detail: >> >> >> >> Total number of spectra in library: 2879 >> >> Total number of distinct peptide ions in library: 2349 >> >> Total number of distinct stripped peptides in library: 2298 >> >> >> >> CHARGE +1: 0 ; +2: 2098 ; +3: 781 ; +4: 0 ; +5: 0 ; >+5: 0 ; >> Unk: 0 >> >> TERMINI Tryptic: 2404 ; Semi-tryptic: 462 ; Non-tryptic: 13 >> >> PROBABILITY >0.9999: 61 ; 0.999-0.9999: 1236 ; 0.99-0.999: 936 ; >> 0.9-0.99: 421 ; <0.9: 225 >> >> NREPS 20+: 0 ; 10-19: 0 ; 4-9: 0 ; 2-3: 0 ; 1: 2879 >> >> MODIFICATIONS E,Glu->pyro-Glu: 215 >> >> K,TMT6plex: 197 >> >> Q,Gln->pyro-Glu: 101 >> >> n,TMT6plex: 2879 >> >> >> >> >> >> On Wednesday, August 31, 2016 at 8:48:09 PM UTC-4, Ali wrote: >> >> Hi >> >> >> >> I want to create a library from a pep.xml file which is the result of X! >> Tandem search (I converted the X!Tandem to pep.xml using Tandem2XML) using >> spectrast with the following simple command: >> >> >> >> spectrast -cN1607011A_NC20A_10ul_180min.splib -cP0.9 >> 1607011A_NC20A_10ul_180min.tandem.pep.xml >> >> >> >> I receive the following error: >> >> >> >> SpectraST finished at Wed Aug 31 15:08:36 2016 with 195 error(s): >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]CPRAAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.02812.02812.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MAAAAAQGGGGGEPR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.02849.02849.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MSGGGGGGGSAPSR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.03003.03003.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGGGQR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.03085.03085.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGLLGR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.03108.03108.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGGGQR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.03354.03354.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MAAAAGGGSCPGPGSAR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.03413.03413.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MATANGAVENGQPDR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.03452.03452.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGLVAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.03464.03464.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]LAGGVR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.03465.03465.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MSVQVAAPGSAGLGPER". Skipped spectrum >> 1607011A_NC20A_10ul_180min.03771.03771.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]DAGSER". >> Skipped spectrum 1607011A_NC20A_10ul_180min.03826.03826.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]LAGGVR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.03846.03846.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]IIGPRGPGSQR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.03857.03857.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]RRPRQGGGGAGGSAAAR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.03870.03870.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]APGVAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.03873.03873.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]PPCSGGDGSTPPGPSLR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.03903.03903.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MNNAGHGPTRR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.03906.03906.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]VSSGCR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.04014.04014.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]EDAAAPGR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.04033.04033.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]ATGGDDPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.04047.04047.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]DGGAASQDESSAAAAAAADSR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.04240.04240.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]ACAGSTR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.04383.04383.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MGGGGSALR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.04389.04389.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]LASGPAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.04425.04425.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]DASGRAAAEGWR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.04557.04557.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MHPAGLAAAAAGTPR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.04586.04586.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]PEAGATLR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.04738.04738.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MGTAAAAAAAAAAAAAGEGAR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.05039.05039.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAAGPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.05094.05094.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]PGGPSPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.05100.05100.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]ERGGAGGGPGGAGGGSGQR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.05205.05205.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]ERGGAGGGPGGAGGGSGQR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.05214.05214.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]ERGGAGGGPGGAGGGSGQR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.05221.05221.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]ERGGAGGGPGGAGGGSGQR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.05235.05235.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]GTAAAAAAAAAAAAAGEGAR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.05378.05378.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GAAGSDGR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.05586.05586.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MSVQVAAPGSAGLGPER". Skipped spectrum >> 1607011A_NC20A_10ul_180min.05662.05662.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAPPAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.05706.05706.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAPVAAGSGAGR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.05716.05716.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MAAAAGGGSCPGPGSAR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.05833.05833.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MGSVGSQR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06095.06095.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAAAQSR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06135.06135.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGSGAGVR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06378.06378.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MDSGAGGRR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06449.06449.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MGRAAATAGGGGGARR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.06460.06460.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MSSGGGSR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06471.06471.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]DPAPAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06521.06521.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MGPGPSR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06526.06526.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SSCSGLSR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06577.06577.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]PGGAGAAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06584.06584.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GFGPGLTAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06635.06635.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MEPGSAEMPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06742.06742.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MRSGAAPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06824.06824.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SGAAGAAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06871.06871.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]AAAAAGLGGGGAGPGPEAGDFLAR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.06879.06879.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GAAAESGR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06903.06903.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAVAAALAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06951.06951.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAIPALD". >> Skipped spectrum 1607011A_NC20A_10ul_180min.06954.06954.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGTTSTRR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.07004.07004.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAGAAPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.07207.07207.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MASDAVQSEPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.07208.07208.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MAAPAGGGGSAVSVLAPNGR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.07211.07211.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]FGGPGPGVLGAQGMAGPLR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.07272.07272.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]RPLAGGSR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.07288.07288.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SGAAGAAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.07350.07350.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MGGCAGSR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.07380.07380.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]PSGEER". >> Skipped spectrum 1607011A_NC20A_10ul_180min.07404.07404.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MSAPVGPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.07444.07444.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]ATSGDCPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.07583.07583.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MGPGPSR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.07610.07610.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAEGGFD". >> Skipped spectrum 1607011A_NC20A_10ul_180min.07708.07708.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AVCGGAAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.07724.07724.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]VSVPGPAAAAAPAAGR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.07838.07838.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SPAAAAAGAGER". >> Skipped spectrum 1607011A_NC20A_10ul_180min.07988.07988.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MSPGASR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08063.08063.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAGAGAPGNPED". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08072.08072.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAGAGAPGNPED". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08094.08094.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]DGVAGPQLGAAAEAAEAAEAR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.08098.08098.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGAWGR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08180.08180.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MPGEAAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08206.08206.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]AAPYPGSGGGSEVK[357]". Skipped spectrum >> 1607011A_NC20A_10ul_180min.08321.08321.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MAVAVGVRAAPVLGLAR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.08406.08406.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGSGAGVR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08430.08430.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGSGAGVR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08433.08433.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SPEAQPQR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08477.08477.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SGFSTEER". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08492.08492.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GNAPSQD". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08511.08511.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGGVCVGR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08531.08531.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGSGAGVR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08548.08548.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAAVAGMLR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08552.08552.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]EAAVGVPDGGDQGGAGPR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.08642.08642.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAAGGAASR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08768.08768.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]ALNVAPVR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08854.08854.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MAAAEEGCSVGAEADR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.08971.08971.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]PGILVGGAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.08998.08998.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAAGAAVAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09035.09035.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MHGGGPPSGDSACPLR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.09094.09094.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]AAQGVGPGPGSAAPPGLEAAR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.09099.09099.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAAAGGAASR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09119.09119.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SGAAGAAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09199.09199.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAAEER". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09255.09255.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]PGILVGGAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09269.09269.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGAWGR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09348.09348.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]TGGAHSR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09380.09380.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]PNCSCAAGVSCTCASSCK[357]". Skipped spectrum >> 1607011A_NC20A_10ul_180min.09414.09414.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GPVPSR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09426.09426.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MPGGPSPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09537.09537.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGAWGR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09639.09639.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]AGPGSTGGQIGAAALAGGAR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.09659.09659.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGSGAGVR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09694.09694.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]GGGPSGGGPGGSGR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.09722.09722.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]GGGPSGGGPGGSGR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.09723.09723.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAGGSAPEPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09727.09727.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MGGEQEEER". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09733.09733.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAGGSAPEPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09746.09746.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAGGSAPEPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09782.09782.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGSGAGVR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09801.09801.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAGGSAPEPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09855.09855.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GVSALLAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.09869.09869.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]GGGPSGGGPGGSGR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.09906.09906.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SGAGGRR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.10082.10082.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]AAAAAAAAAAGAAGGR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.10143.10143.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]AAAAAAAAAAGAAGGR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.10163.10163.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]AAAAAAAAAAGAAGGR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.10324.10324.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]AAAAAAAAAAGAAGGR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.10369.10369.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MPGPPALR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.10601.10601.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]GGGPSGGGPGGSGR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.10854.10854.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]GGGPSGGGPGGSGR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.10855.10855.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MASEASVR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.10910.10910.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]GGGPSGGGPGGSGR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.10933.10933.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAAVGSGGYAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.10976.10976.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MSSEDR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.10991.10991.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]SGAAGAAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.11115.11115.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MASATED". >> Skipped spectrum 1607011A_NC20A_10ul_180min.11259.11259.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]ASVAAAARR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.11363.11363.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AELGAGGDGHR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.11367.11367.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AELGAGGDGHR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.11369.11369.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MEEGAPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.11427.11427.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGSDPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.11445.11445.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]FLLGLR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.11584.11584.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]AGGGGGSSK[357]". Skipped spectrum >> 1607011A_NC20A_10ul_180min.11673.11673.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]LPPPPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.11826.11826.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGSDPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.11957.11957.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]ELGAGGDGHR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.12159.12159.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]GAAGAGAPGNPED". Skipped spectrum >> 1607011A_NC20A_10ul_180min.12280.12280.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MELGGPGAPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.12308.12308.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGAWGR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.12420.12420.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MELGGPGAPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.12496.12496.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGGSDPR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.12559.12559.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MASASGAMAK[357]". Skipped spectrum >> 1607011A_NC20A_10ul_180min.12580.12580.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GRAGGGGPGR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.12788.12788.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MSELADSGPSGASAPSQLR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.12809.12809.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GSGGGAGER". >> Skipped spectrum 1607011A_NC20A_10ul_180min.13091.13091.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MATSGANGPGSATASASNPR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.13144.13144.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]ANGDSPPWSPALAAEGRGSSCEVR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.13183.13183.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MAGAGSEAR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.13366.13366.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]RSPGGILLQALPR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.13403.13403.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MGVALR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.13432.13432.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MATSFR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.13484.13484.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MTEPGASPED". >> Skipped spectrum 1607011A_NC20A_10ul_180min.13555.13555.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MTEPGASPED". >> Skipped spectrum 1607011A_NC20A_10ul_180min.13607.13607.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GGAGGGPGLR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.13613.13613.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]MTEPGASPED". >> Skipped spectrum 1607011A_NC20A_10ul_180min.13725.13725.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]AALHTTPDSPAAQLER". Skipped spectrum >> 1607011A_NC20A_10ul_180min.13774.13774.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AAAGPSTR". >> Skipped spectrum 1607011A_NC20A_10ul_180min.13888.13888.2 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]RSGGGCSGSLSLR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.14320.14320.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]ATVVDAVNAAPLSGSK[357]". Skipped spectrum >> 1607011A_NC20A_10ul_180min.14447.14447.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MEAAFGQVAGSACPRR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.14956.14956.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MAASAGAGAVIAAPDSR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.15253.15253.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MGGAGALGGAGGVQGAAGR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.15317.15317.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]TTFLQTSSSTFGGGSTR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.15616.15616.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MLPWTALGLALSLRLALAR". Skipped spectrum >> 1607011A_NC20A_10ul_180min.16176.16176.3 . >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MASGAGGVGGGGGGK[357]" >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MVK[357]EGAAVIDVGINYV >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MRQGLLVLALVLVLVLVLAAG >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]AAIYGGVEGGGTR". Skipp >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]LGSDDFFYVGGSPSTADLPGS >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]EGGPAVCCQDPRAELVER". >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]AAAAMAAAAGGGAGAARSLSR >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]LNGGGSGAGGSRGGGQER". >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]RRALLLLLLSFLAPWATIALR >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]APGALAQTAAPGPGR". Ski >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MAGDLSAGFFMEELNTYR". >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]GAAARLSAPR". >> Skipped >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]SSYDEASLAPEETTDSFWEVG >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]APRALPGSAVLAAAVFVGGAV >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MSHYGSYYGGLGYSCGGFGGL >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MDGASSGGGGSSEGGGGSSGS >> >> PEPXML IMPORT: Peptide ID has unknown modification: "n[272]AGAAAGGR". >> Skipped sp >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]SCARLLLLWGCTVVAAGLSGV >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]ASTSRDVIAGRGIHSK[357] >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]TESASCSTARQTK[357]". >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]MARAGWTGLLPLYVCLLLTCG >> >> PEPXML IMPORT: Peptide ID has unknown modification: >> "n[272]AAAAPAAAAASSEAPAASATA >> >> >> >> >> >> Does any body knows what might be the problem? I am relatively new in TPP >> and proteomics so I would appreciate if you could explain a little in >> detail. >> >> >> >> Thank you very much >> >> >> >> -- >> You received this message because you are subscribed to the Google Groups >> "spctools-discuss" group. >> To unsubscribe from this group and stop receiving emails from it, send an >> email to [email protected]. >> To post to this group, send email to [email protected]. >> Visit this group at https://groups.google.com/group/spctools-discuss. >> For more options, visit https://groups.google.com/d/optout. >> > -- You received this message because you are subscribed to the Google Groups "spctools-discuss" group. To unsubscribe from this group and stop receiving emails from it, send an email to [email protected]. To post to this group, send email to [email protected]. Visit this group at https://groups.google.com/group/spctools-discuss. For more options, visit https://groups.google.com/d/optout.
