Many thanks for your response David!

I've read the page you mentioned, I wanted to make sure.

Sincerely,

Ali

On Monday, September 5, 2016 at 12:24:47 PM UTC-4, David Shteynberg wrote:
>
> Hello Ali,
>
> For more information on SpectraST please see the following: 
> http://tools.proteomecenter.org/wiki/index.php?title=Software:SpectraST
>
>
> A quick search for retention time on that page suggest retention times are 
> used for normalization using landmark peptides (e.g. iRT).  So I believe 
> the short answer to your question is no. 
>
> That said, the RT model in PeptideProphet can use retention times to help 
> promote peptide IDs that elute near their expected RT and demote those 
> elute not near expectation.
>
> -David
>
>
> On Sun, Sep 4, 2016 at 6:30 PM, Ali <[email protected] <javascript:>> 
> wrote:
>
>> Hi
>>
>> Does any body know whether SpectraST uses retention time in analyzing a 
>> shotgun data (a spectrast search) or not? Can I get some details about it?
>>
>> Thank you very much,
>>
>> *Ali*
>>
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