The version of the software you are using is old.  Please consider trying
the latest version available, 5.0.0, as it provides many bug fixes and
enhancements.  Let us know if you still have this problem with the latest
version.

Cheers,
-David

On Dec 16, 2016 11:32 AM, "HFaz" <[email protected]> wrote:

> I get the following error when i tried to use tandem to search my Ecoli
> sample. I appreciate any idea of why this is happening and how to resolve
> it.
>
> *EXECUTING: cd c:/Inetpub/wwwroot/ISB/data/Ecoli&
> c:\Inetpub\tpp-bin\xinteract -Ninteract.pep.xml -p0.05 -l7 -PPM -OAPE
> -dDECOY e161212_EC2_03.tandem.pep.xml *
>
> c:\Inetpub\tpp-bin\xinteract (TPP v4.8.0 PHILAE, Build 201411201551-6764 
> (mingw-i686))
>  PPM mode in Accurate Mass Model ...
> The syntax of the command is incorrect.
>
> command "mkdir -p C:/tmp/a20608" failed: Operation not permittedwarning: the 
> webserver temporary directory C:/tmp/a20608 specified by WEBSERVER_TMP does 
> not exist and could not be created.  Tempfiles will created in data 
> directories instead.
> running: "C:/Inetpub/tpp-bin/InteractParser "C:/tmp/a20608/interact.pep.xml" 
> "e161212_EC2_03.tandem.pep.xml" -L"7""
>  file 1: e161212_EC2_03.tandem.pep.xml
> SUCCESS: CORRECTED data file 
> c:/Inetpub/wwwroot/ISB/data/Ecoli/e161212_EC2_03.mzXML in msms_run_summary 
> tag ...
>  processed altogether 11459 results
> INFO: Results written to file: c:/tmp/a20608/interact.pep.xml
> command completed in 3 sec
>
> running: "C:/Inetpub/tpp-bin/DatabaseParser "C:/tmp/a20608/interact.pep.xml""
> pipe "C:/Inetpub/tpp-bin/DatabaseParser "C:/tmp/a20608/interact.pep.xml"" 
> failed: No errorerror, could not execute: C:/Inetpub/tpp-bin/DatabaseParser 
> "C:/tmp/a20608/interact.pep.xml"
>
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