This seems like an installation issue.  Do you have a version of Perl
installed on your system?  As of 5.0, TPP ships with its own Perl language
for easy installation, but it may clash if Perl already exists on your
system.  The easiest solution is to remove/uninstall Perl (if you do not
need it, of course), and try running TPP again.

Hope this helps,
--Luis


On Mon, May 22, 2017 at 1:36 PM, Aeon K <[email protected]> wrote:

> Sorry if this problem had already been solved, but I can´t find nothing at
> the spctools...
>
> I´m dealing with this problem at output files:
>
>    - c:/TPP/data/interact.prot.xml (*file not found:* not created or
>    deleted?)
>
> And on the command section is this what´s happening:
>
> running: "C:/TPP/bin/PeptideProphetParser "interact.pep.xml" MINPROB=0.05 
> PPM"using PPM mass difference
>  (X! Tandem (k-score))
> init with X! Tandem (k-score) trypsin
> MS Instrument info: Manufacturer: Thermo Scientific, Model: LTQ Orbitrap 
> Velos, Ionization: nanoelectrospray, Analyzer: radial ejection linear ion 
> trap, Detector: electron multiplier
>
> INFO: Processing standard MixtureModel ...
>  PeptideProphet  (TPP v5.0.0 Typhoon, Build 201610111222-7423 
> (Windows_NT-x86_64)) AKeller@ISB
>  read in 0 1+, 2517 2+, 1606 3+, 307 4+, 45 5+, 0 6+, and 0 7+ spectra.
> Initialising statistical models ...
> negmean = 0.0533258
> negmean = 0.304483
> negmean = -0.455953
> negmean = 0.717048
> negmean = 0.996458
> negmean = -0.2
> negmean = -0.2
> Iterations: .........10.........20.........30WARNING: Mixture model quality 
> test failed for charge (5+).
> model complete after 31 iterations
> command completed in 1 sec
> running: "C:/TPP/bin/ProphetModels.pl -i interact.pep.xml"Can't locate 
> FindBin/libs.pm in @INC (you may need to install the FindBin::libs module) 
> (@INC contains: C:/TPP/lib/perl C:/Perl64/site/lib C:/Perl64/lib .) at 
> C:/TPP/bin/ProphetModels.pl line 33.
> BEGIN failed--compilation aborted at C:/TPP/bin/ProphetModels.pl line 33.
>
> command "perl C:/TPP/bin/ProphetModels.pl -i interact.pep.xml" failed: No 
> such file or directory
>
> command "C:/TPP/bin/ProphetModels.pl -i interact.pep.xml" exited with 
> non-zero exit code: 2
> QUIT - the job is incomplete
>
>
> Some help please....  :)
>
>
> --
> You received this message because you are subscribed to the Google Groups
> "spctools-discuss" group.
> To unsubscribe from this group and stop receiving emails from it, send an
> email to [email protected].
> To post to this group, send email to [email protected].
> Visit this group at https://groups.google.com/group/spctools-discuss.
> For more options, visit https://groups.google.com/d/optout.
>

-- 
You received this message because you are subscribed to the Google Groups 
"spctools-discuss" group.
To unsubscribe from this group and stop receiving emails from it, send an email 
to [email protected].
To post to this group, send email to [email protected].
Visit this group at https://groups.google.com/group/spctools-discuss.
For more options, visit https://groups.google.com/d/optout.

Reply via email to